Effect of differential exposure of UV-C and melatonin stress on the therapeutic potential of bioactive compounds in Moringa oleifera

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-10-24 DOI:10.1016/j.sajb.2024.10.024

Muhammad Naeem Bajwa , Gouhar Zaman , Muhammad Asad Ullah , Muhammad Uzair Javed , Anisa Andleeb , Christophe Hano , Bilal Haider Abbasi

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Abstract

Introduction

Moringa oleifera, widely recognized as a miracle tree, boasts a rich repository of bioactive metabolites and nutraceuticals. This study explores a biotechnological approach utilizing UV-C and melatonin elicitation for enhancing pharmaceutically significant secondary metabolites in M. oleifera callus cultures.

Methods

callus cultures were exposed to different UV-C exposure durations and various melatonin concentrations to assess their combined impacts on biomass, secondary metabolite synthesis, and antioxidant ability. Biomass yields for fresh weight (F.W) were measured for 10 μM melatonin (176. 18 g/L) and UV-C 60 min (172.17 g/L). Total phenolic production (TPP: 25.83 mg/L D.W) and total flavonoids production (TFP: 157.07 mg/L D.W) peaked at 15 μM melatonin. High-performance liquid chromatography facilitated metabolite quantification.

Results

Significant increases in total phytochemical production were observed, reaching 12.98 mg g^-1 D.W with 15 μM melatonin—an approximately 2.5-fold rise. UV-C exposure for 60 min yielded a two-fold increase (10.847 mg g^-1 D.W) compared to the control (5.155 mg g^-1 D.W). Metabolic profiling identified four phytochemicals (p-coumaric acid, chlorogenic acid, rutin, and apigenin) through HPLC. Melatonin at 15 μM demonstrated superior DPPH free radical scavenging activity (93.32 %) compared to the control (82.05 %). Additionally, FRAP (382.52 TAEC μM) and ABTS (535.8 TAEC μM) exhibited maximum values under 15 μM melatonin concentration.

Conclusions

This study provides a comprehensive approach for enhanced phytochemical production, offering insights into the comparative impacts of UV-C and melatonin elicitation on secondary metabolite pathways in M. oleifera callus cultures.

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紫外线-C 和褪黑激素应激的不同暴露对油辣木生物活性化合物治疗潜力的影响

引言油橄榄被广泛认为是一种神奇的树木，拥有丰富的生物活性代谢物和营养保健品。本研究探索了一种生物技术方法，即利用紫外线-C 和褪黑激素诱导来提高油橄榄胼胝体培养物中具有重要药用价值的次生代谢物。方法将胼胝体培养物暴露于不同的紫外线-C 暴露持续时间和不同浓度的褪黑激素，以评估它们对生物量、次生代谢物合成和抗氧化能力的综合影响。测定了 10 μM 褪黑激素（176.18 克/升）和紫外线-C 60 分钟（172.17 克/升）的生物量产量（鲜重）。总酚产量（TPP：25.83 mg/L D.W）和总黄酮产量（TFP：157.07 mg/L D.W）在 15 μM 褪黑激素时达到峰值。结果观察到植物化学物质总产量显著增加，15 μM 褪黑激素时达到 12.98 mg g-1 D.W，大约增加了 2.5 倍。与对照组（5.155 mg g-1 D.W）相比，紫外线-C 照射 60 分钟产生的植物化学物质增加了两倍（10.847 mg g-1 D.W）。通过高效液相色谱法，代谢分析确定了四种植物化学物质（对香豆酸、绿原酸、芦丁和芹菜素）。与对照组（82.05%）相比，15 μM 的褪黑素具有更高的 DPPH 自由基清除活性（93.32%）。此外，在 15 μM 的褪黑激素浓度下，FRAP（382.52 TAEC μM）和 ABTS（535.8 TAEC μM）显示出最大值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464