Genome-wide identification of TCP transcription factors and functional role of UrTCP4 in regulating terpenoid indole alkaloids biosynthesis in Uncaria rhynchophylla

IF 5.4 Q1 PLANT SCIENCES
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Abstract

Uncaria rhynchophylla is a widely used Chinese herbal medicine known for its terpenoid indole alkaloids (TIAs), which help in treating hypertension. Teosinte branched 1/cycloidea/proliferating cell factors 1/2 (TCP) TFs have been shown to have a role in the growth and development of plants, but the regulation mechanism of UrTCP in the TIAs biosynthesis pathway is yet unknown. In this study, twenty-six UrTCP genes were identified from the genome of U. rhynchophylla, and these genes were classified into three subgroups based on the phylogenetic analysis. UrTCPs from the same group or subgroup share comparable gene structures and conserved motifs. These 26 UrTCP genes were unevenly distributed throughout 22 chromosomes, and gene segmental duplication occurred. The interspecific co-linearity analysis suggested that UrTCP4 may have an important function in evolutionary process. The expression patterns analysis of UrTCP genes by RT-qPCR showed most UrTCP genes had the highest expression in leaves and stem hooks. The results of co-expression analysis and phylogenetic relationships screened UrTCP4 as the most likely participant in TIAs and its precursor synthesis. Furthermore, the subcellular localization of UrTCP4 indicated that it is located in nucleus. Dual luciferase assays revealed the UrTCP4 protein could activate or repress the transcription of UrLAMT and Ur7-DLH in the TIAs synthesis pathway. Finally, Yeast-one-hybrid assays demonstrated that the UrTCP4 protein can bind to the promoters of UrLAMT and Ur7-DLH. Thus, UrTCP4 may be involved in the regulation of the TIAs biosynthesis pathway. This research establishes a groundwork for elucidating the functions of UrTCP gene family in U. rhynchophylla, offering new insights into the regulation of TIAs.
全基因组范围内 TCP 转录因子的鉴定以及 UrTCP4 在调控 Uncaria rhynchophylla 中萜类吲哚生物碱生物合成中的功能作用
云芝(Uncaria rhynchophylla)是一种广泛使用的中药,因其萜类吲哚生物碱(TIAs)而闻名,TIAs有助于治疗高血压。Teosinte支链1/cycloidea/增殖细胞因子1/2(TCP)TFs已被证明在植物的生长发育中发挥作用,但UrTCP在TIAs生物合成途径中的调控机制尚不清楚。本研究从 U. rhynchophylla 的基因组中鉴定出 26 个 UrTCP 基因,并根据系统进化分析将这些基因分为三个亚组。同组或亚组中的 UrTCPs 具有相似的基因结构和保守基序。这 26 个 UrTCP 基因不均匀地分布在 22 条染色体上,且存在基因片段重复现象。种间共线性分析表明,UrTCP4在进化过程中可能具有重要功能。通过 RT-qPCR 对 UrTCP 基因的表达模式进行分析,发现大多数 UrTCP 基因在叶片和茎钩中的表达量最高。共表达分析和系统进化关系的结果筛选出 UrTCP4 最有可能参与 TIAs 及其前体的合成。此外,UrTCP4 的亚细胞定位表明它位于细胞核中。双荧光素酶试验显示,UrTCP4 蛋白可激活或抑制 TIAs 合成途径中 UrLAMT 和 Ur7-DLH 的转录。最后,酵母一杂交试验证明,UrTCP4 蛋白能与 UrLAMT 和 Ur7-DLH 的启动子结合。因此,UrTCP4可能参与了TIAs生物合成途径的调控。这项研究为阐明 U. rhynchophylla 中 UrTCP 基因家族的功能奠定了基础,为 TIAs 的调控提供了新的见解。
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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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