Effect of supercritical CO2 extraction as pretreatment to obtain C-phycocyanin from spirulina (Arthrospira maxima)

IF 3.4 3区工程技术 Q2 CHEMISTRY, PHYSICAL

Journal of Supercritical Fluids Pub Date : 2024-10-16 DOI:10.1016/j.supflu.2024.106428

J.A. López-Limón , A.S. Hernández-Cázares , J.V. Hidalgo-Contreras , G. Romero - De la Vega , R.A. Mellado-Pumarino , M.A. Ríos-Corripio

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Abstract

C-phycocyanin (CPC) is a phycobiliprotein present in spirulina, which, due to its blue color, non-toxic nature, fluorescent and antioxidant properties, is of interest in the food and pharmaceutical industries. Different methods of extraction and stabilization are being investigated, including supercritical CO₂ extraction, a method applied to obtain bioactive compounds from plant matrices. In this work, the effect of applying supercritical CO₂ as pretreatment for the extraction of CPC at different parameters (pressure, temperature, use of cosolvent) was evaluated. Best pretreatment conditions to obtain the highest purity were at 17 MPa and 45 °C, considering it reagent grade. When comparing UV and FTIR spectra of the raffinates, a similarity was obtained with the analytical standard, with the presence of a band at 620 nm in UV and for FTIR a band at 1650 and 1540 cm⁻¹ that corresponds to amide I and amide II, corroborating their presence and the structural characteristics.

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超临界二氧化碳萃取作为从螺旋藻（Arthrospira maxima）中获取 C-花青素的预处理的效果

C-phycocyanin (CPC) 是螺旋藻中的一种藻胆蛋白，由于它呈蓝色、无毒、具有荧光和抗氧化特性，因此受到食品和制药行业的关注。目前正在研究不同的提取和稳定方法，包括超临界二氧化碳萃取法，这是一种用于从植物基质中获取生物活性化合物的方法。在这项工作中，我们评估了在不同参数（压力、温度、共溶剂的使用）下将超临界 CO2 作为提取 CPC 的预处理的效果。获得最高纯度的最佳预处理条件是在 17 兆帕和 45 °C（考虑到其为试剂级）。在比较酒石酸盐的紫外光谱和傅立叶变换红外光谱时，发现它们与分析标准相似，紫外光谱在 620 nm 处有一条带，傅立叶变换红外光谱在 1650 和 1540 cm-1 处有一条带，分别对应于酰胺 I 和酰胺 II，证实了它们的存在和结构特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Supercritical Fluids 工程技术-工程：化工

CiteScore

7.60

自引率

10.30%

发文量

236

审稿时长

56 days

期刊介绍： The Journal of Supercritical Fluids is an international journal devoted to the fundamental and applied aspects of supercritical fluids and processes. Its aim is to provide a focused platform for academic and industrial researchers to report their findings and to have ready access to the advances in this rapidly growing field. Its coverage is multidisciplinary and includes both basic and applied topics. Thermodynamics and phase equilibria, reaction kinetics and rate processes, thermal and transport properties, and all topics related to processing such as separations (extraction, fractionation, purification, chromatography) nucleation and impregnation are within the scope. Accounts of specific engineering applications such as those encountered in food, fuel, natural products, minerals, pharmaceuticals and polymer industries are included. Topics related to high pressure equipment design, analytical techniques, sensors, and process control methodologies are also within the scope of the journal.