Dharam Pal, Subodh Kumar, Subhash Chander Bhardwaj, Om Prakash Gangwar, Anjali Pal, Madhu Patial, Santosh Watpade, Harikrishna, Niharika Mallick, Vikas Fandade, J. K. Roy
{"title":"Identification of Rust Resistance Genes in Wheat (Triticum aestivum L.) Using Molecular Markers and Host–Pathogen Interaction Tests","authors":"Dharam Pal, Subodh Kumar, Subhash Chander Bhardwaj, Om Prakash Gangwar, Anjali Pal, Madhu Patial, Santosh Watpade, Harikrishna, Niharika Mallick, Vikas Fandade, J. K. Roy","doi":"10.1111/jph.13417","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>The leaf rust (<i>Puccinia triticina</i> f. sp. <i>tritici</i>), stripe rust (<i>Puccinia striiformis</i> f. sp. <i>tritici</i>), and stem rust (<i>Puccinia graminis</i> f. sp. <i>tritici</i>) are major fungal constraints affecting wheat production worldwide. Identifying and deploying wheat varieties with diverse resistance are the best ways to manage all the rusts. Therefore, a continuous search goes on to identify diverse germplasm with effective rust resistance that expresses at different stages of plant growth (seedling and adult plant). A set of 22 rust resistant wheat genotypes and 4 checks (controls), viz., Avocet-Yr10, Avocet -Yr15, Agra Local, and respective positive checks were studied for characterising rust resistance genes using host–pathogen interactions complemented by molecular markers. Among 22 elite genotypes, 05 genotypes amplified 191 bp fragment with marker <i>PSY1E1</i>, confirmed the presence of gene <i>Lr19</i>/<i>Sr25</i>. These genotypes also expressed resistance to most virulent leaf rust pathotypes, 77-5 and 77-9 in host–pathogen interaction test (HPI). Seven genotypes showed the presence of <i>Lr34/Yr18/Sr57/Pm38/Ltn1</i> in homozygous state, whereas G4 showed its presence in heterozygous condition. Among 22 genotypes, 16 genotypes possessed <i>Yr10</i>. Five genotypes (22.7%) exhibited two gene combinations, <i>Lr19/Sr25</i>, and <i>Yr10</i> as revealed through the detection of 191 bp fragment with marker <i>PSY1E1</i> and 260 bp fragment with co-dominantly inherited microsatellite marker <i>Xpsp3000</i>, respectively. All five genotypes (G2, G3, G8, G9, and G18) also expressed brown glumes controlled by the gene <i>Rg1</i> tightly linked to <i>Yr10</i> on the 1BS chromosome. Broad spectrum rust resistance present in these lines in good agronomic backgrounds could be used as potent genetic donors for diverse and durable rust resistance breeding programmes in wheat.</p>\n </div>","PeriodicalId":16843,"journal":{"name":"Journal of Phytopathology","volume":"172 5","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jph.13417","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The leaf rust (Puccinia triticina f. sp. tritici), stripe rust (Puccinia striiformis f. sp. tritici), and stem rust (Puccinia graminis f. sp. tritici) are major fungal constraints affecting wheat production worldwide. Identifying and deploying wheat varieties with diverse resistance are the best ways to manage all the rusts. Therefore, a continuous search goes on to identify diverse germplasm with effective rust resistance that expresses at different stages of plant growth (seedling and adult plant). A set of 22 rust resistant wheat genotypes and 4 checks (controls), viz., Avocet-Yr10, Avocet -Yr15, Agra Local, and respective positive checks were studied for characterising rust resistance genes using host–pathogen interactions complemented by molecular markers. Among 22 elite genotypes, 05 genotypes amplified 191 bp fragment with marker PSY1E1, confirmed the presence of gene Lr19/Sr25. These genotypes also expressed resistance to most virulent leaf rust pathotypes, 77-5 and 77-9 in host–pathogen interaction test (HPI). Seven genotypes showed the presence of Lr34/Yr18/Sr57/Pm38/Ltn1 in homozygous state, whereas G4 showed its presence in heterozygous condition. Among 22 genotypes, 16 genotypes possessed Yr10. Five genotypes (22.7%) exhibited two gene combinations, Lr19/Sr25, and Yr10 as revealed through the detection of 191 bp fragment with marker PSY1E1 and 260 bp fragment with co-dominantly inherited microsatellite marker Xpsp3000, respectively. All five genotypes (G2, G3, G8, G9, and G18) also expressed brown glumes controlled by the gene Rg1 tightly linked to Yr10 on the 1BS chromosome. Broad spectrum rust resistance present in these lines in good agronomic backgrounds could be used as potent genetic donors for diverse and durable rust resistance breeding programmes in wheat.
期刊介绍:
Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays.
Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes.
Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.