When are you measuring soil β-glucosidase activities in cropping systems?

Abstract

In situ soil respiration is driven by annual patterns of temperature and soil moisture, but what about extracellular enzyme activities responsible for depolymerizing soil organic matter? We conducted biweekly measurements of potential soil β-glucosidase activities during a 4-month period from March soil thawing through July in annually cropped field plots in eastern South Dakota. Our objective was to determine the best sampling time to resolve the effects of crop rotational diversity on soil microbial activities. Potential β-glucosidase activities were elevated immediately following soil thaw, peaked in May, and declined to their lowest value in mid-summer. Temperature and precipitation had no value in predicting enzyme activities; however, enzyme activities were affected by crop rotational diversity and responded to current crop and previous crop. These findings are pertinent to the use of soil extracellular enzymes in soil health assessments and as indicators of microbial substrate preference with implications for soil carbon stabilization.