Production and characterization of bacterial cellulose synthesized by Enterobacter chuandaensis strain AEC using Phoenix dactylifera and Musa acuminata

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Ashraf Sami Hassan AL-Hasabe, Ahmad Faizal Bin Abdull Razis, Nadiya Akmal Binti Baharum, Choo Yee Yu, Nurulfiza Mat Isa
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Abstract

Bacterial cellulose (BC) is a biopolymer synthesized extracellularly by certain bacteria through the polymerization of glucose monomers. This study aimed to produce BC using Enterobacter chuandaensis with fruit extracts from Phoenix dactylifera (D) and Musa acuminata (M) as carbon sources. Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) showed characteristic cellulose vibrations, while X-ray diffraction (XRD) identified distinct peaks at 15.34°, 19.98°, 22.58°, and 34.6°, confirming the cellulose structure. Whole-genome sequencing of E. chuandaensis identified key genes involved in BC production. The BC produced then exhibited a molecular weight of 1,857,804 g/mol, with yields of 2.8 g/L and 2.5 g/L for treatments D and M, respectively. The crystallinity index of the purified BC was 74.1, and 13C NMR analysis confirmed the dominant cellulose Iα crystalline form. The BC showed high biocompatibility in cytotoxicity assays, with cell viability between 92% and 100%, indicating its potential for use in biomedical applications. This investigation represents the first report of BC production by E. chuandaensis, which promises a new avenue for sustainable and efficient BC synthesis using fruit extracts as carbon sources.

川达肠杆菌 AEC 菌株利用凤凰木和尖叶麝香合成的细菌纤维素的生产和特性分析
细菌纤维素(BC)是由某些细菌通过聚合葡萄糖单体在细胞外合成的一种生物聚合物。本研究旨在利用川达肠杆菌,以凤凰果(D)和尖叶麝香(M)的果实提取物为碳源,生产生物纤维素。衰减全反射-傅立叶变换红外光谱(ATR-FTIR)显示了纤维素的特征振动,而 X 射线衍射(XRD)在 15.34°、19.98°、22.58° 和 34.6°处发现了明显的峰值,证实了纤维素结构。E. chuandaensis 的全基因组测序确定了参与生产 BC 的关键基因。随后生产的 BC 分子量为 1,857,804 g/mol,处理 D 和处理 M 的产量分别为 2.8 g/L 和 2.5 g/L。纯化 BC 的结晶度指数为 74.1,13C NMR 分析证实其主要是纤维素 Iα 结晶形式。在细胞毒性实验中,这种 BC 表现出很高的生物相容性,细胞存活率在 92% 到 100% 之间,这表明它具有生物医学应用的潜力。这项研究首次报道了E. chuandaensis生产BC的情况,为利用水果提取物作为碳源进行可持续、高效的BC合成开辟了一条新途径。
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来源期刊
Archives of Microbiology
Archives of Microbiology 生物-微生物学
CiteScore
4.90
自引率
3.60%
发文量
601
审稿时长
3 months
期刊介绍: Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.
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