Lipase-entrapped colloidosomes with light-responsive wettability for efficient and recyclable Pickering interfacial biocatalysis†

IF 11.3 1区化学 Q1 CHEMISTRY, PHYSICAL

ACS Catalysis Pub Date : 2024-10-01 DOI:10.1039/D4GC03982C

Dingyi Yang, Qi Zeng, Kaiwen Tan, Haoyue Hou, Xingyuan Fang, Chenlong Guo, Hao Yuan and Tao Meng

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Abstract

Light-responsive Pickering interfacial biocatalysis (LPIB) is desirable because of its convenient catalyst recovery, product separation and clean external stimuli trigger. However, the recyclability of LPIB is still limited due to its enzyme inactivation. Herein, lipase-entrapped colloidosomes as particulate emulsifiers and biocatalysts were fabricated using the one-step co-assembly of lipases and TiO₂ nanoparticles (with light-responsive wettability). The obtained LPIB exhibited a 4.31-fold enhancement of enzyme activity compared with the traditional biphasic system. Impressively, the LPIB maintained nearly 90% of its initial enzyme activity even after 20 cycles, which is the highest among the currently reported LPIBs. It is attributed that the TiO₂ nanoparticle layer on the colloidosome surface reflects UV light, thus protecting the enzyme from photodegradation. This green platform can be widely applied to construct recyclable and efficient biphasic biocatalytic systems.

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具有光响应润湿性的脂肪酶包裹胶体，可用于高效、可回收的 Pickering 界面生物催化†。

光响应皮克林界面生物催化（LPIB）具有催化剂回收、产物分离和清洁外部刺激触发的便利性，因此备受青睐。然而，由于酶失活，LPIB 的可回收性仍然受到限制。本文利用脂肪酶和TiO2纳米颗粒（具有光响应润湿性）的一步共组装技术，制备了作为微粒乳化剂和生物催化剂的脂肪酶包裹胶体。与传统的双相体系相比，所获得的 LPIB 的酶活性提高了 4.31 倍。令人印象深刻的是，LPIB 在 20 次循环后仍能保持近 90% 的初始酶活性，这在目前报道的 LPIB 中是最高的。这归功于胶体表面的 TiO2 纳米粒子层能够反射紫外线，从而保护酶免于光降解。这一绿色平台可广泛应用于构建可回收的高效双相生物催化系统。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Catalysis CHEMISTRY, PHYSICAL-

CiteScore

20.80

自引率

6.20%

发文量

1253

审稿时长

1.5 months

期刊介绍： ACS Catalysis is an esteemed journal that publishes original research in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. It offers broad coverage across diverse areas such as life sciences, organometallics and synthesis, photochemistry and electrochemistry, drug discovery and synthesis, materials science, environmental protection, polymer discovery and synthesis, and energy and fuels. The scope of the journal is to showcase innovative work in various aspects of catalysis. This includes new reactions and novel synthetic approaches utilizing known catalysts, the discovery or modification of new catalysts, elucidation of catalytic mechanisms through cutting-edge investigations, practical enhancements of existing processes, as well as conceptual advances in the field. Contributions to ACS Catalysis can encompass both experimental and theoretical research focused on catalytic molecules, macromolecules, and materials that exhibit catalytic turnover.