Salmonella biofilm formation diminishes bacterial proliferation in the C. elegans intestine

IF 5.9 Q1 MICROBIOLOGY
Ines Thiers, Maries Lissens, Hanne Langie, Bram Lories , Hans Steenackers
{"title":"Salmonella biofilm formation diminishes bacterial proliferation in the C. elegans intestine","authors":"Ines Thiers,&nbsp;Maries Lissens,&nbsp;Hanne Langie,&nbsp;Bram Lories ,&nbsp;Hans Steenackers","doi":"10.1016/j.bioflm.2024.100225","DOIUrl":null,"url":null,"abstract":"<div><div>Non-typhoidal <em>Salmonella</em> serovars are a significant global cause of foodborne infections, owing their transmission success to the formation of biofilms. While the role of these biofilms in <em>Salmonella</em>'s persistence outside the host is well understood, their significance during infection remains elusive. In this study, we investigated the impact of <em>Salmonella</em> biofilm formation on host colonization and virulence using the nematode model <em>Caenorhabditis elegans</em>. This infection model enables us to isolate the effect of biofilm formation on gut colonization and proliferation, as no gut microbiome is present and <em>Salmonella</em> cannot invade the intestinal tissue of the nematode. We show that a biofilm-deficient Δ<em>csgD</em> mutant enhances gut proliferation compared to the wild-type strain, while the pathogen's virulence, the host's immune signaling pathways, and host survival remain unaffected. Hence, our work suggests that biofilm formation does not significantly contribute to <em>Salmonella</em> infection in <em>C. elegans.</em> However, complementary assays in higher-order <em>in vivo</em> models are required to further characterize the role of biofilm formation during infection and to take into account the impact of biofilm formation on competition with gut microbiome and epithelial invasion.</div></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":"8 ","pages":"Article 100225"},"PeriodicalIF":5.9000,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11513601/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biofilm","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590207524000509","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Non-typhoidal Salmonella serovars are a significant global cause of foodborne infections, owing their transmission success to the formation of biofilms. While the role of these biofilms in Salmonella's persistence outside the host is well understood, their significance during infection remains elusive. In this study, we investigated the impact of Salmonella biofilm formation on host colonization and virulence using the nematode model Caenorhabditis elegans. This infection model enables us to isolate the effect of biofilm formation on gut colonization and proliferation, as no gut microbiome is present and Salmonella cannot invade the intestinal tissue of the nematode. We show that a biofilm-deficient ΔcsgD mutant enhances gut proliferation compared to the wild-type strain, while the pathogen's virulence, the host's immune signaling pathways, and host survival remain unaffected. Hence, our work suggests that biofilm formation does not significantly contribute to Salmonella infection in C. elegans. However, complementary assays in higher-order in vivo models are required to further characterize the role of biofilm formation during infection and to take into account the impact of biofilm formation on competition with gut microbiome and epithelial invasion.
沙门氏菌生物膜的形成减少了优雅小鼠肠道中细菌的增殖。
非伤寒沙门氏菌血清型是全球食源性感染的一个重要原因,其传播成功的原因在于生物膜的形成。虽然这些生物膜在沙门氏菌宿主外的持续存在中的作用已广为人知,但它们在感染过程中的意义却仍然难以捉摸。在本研究中,我们利用线虫模型秀丽隐杆线虫研究了沙门氏菌生物膜的形成对宿主定殖和毒力的影响。这种感染模型使我们能够分离出生物膜形成对肠道定植和增殖的影响,因为没有肠道微生物群,沙门氏菌无法侵入线虫的肠道组织。我们的研究表明,与野生型菌株相比,生物膜缺陷ΔcsgD突变体能增强肠道增殖,而病原体的毒力、宿主的免疫信号通路和宿主存活率则不受影响。因此,我们的研究结果表明,生物膜的形成并不会对 elegans 感染沙门氏菌产生重大影响。然而,要进一步确定生物膜形成在感染过程中的作用,并考虑到生物膜形成对肠道微生物群竞争和上皮侵袭的影响,还需要在更高阶的体内模型中进行补充实验。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Biofilm
Biofilm MICROBIOLOGY-
CiteScore
7.50
自引率
1.50%
发文量
30
审稿时长
57 days
期刊介绍:
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信