Exploration of Some Nutrient Absorption-Related Genes and Intestinal Histology in Japanese Quails (Coturnix japonica) Throughout Their Productive Lifespan.

IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES
Hamid Reza Rafieian-Naeini, Mahdi Zhandi, Maryam Taghipour-Shahbandi, Amir Reza Sabzian, Ali Reza Yousefi, Mahboubeh Khajehnabi, Woo Kyun Kim
{"title":"Exploration of Some Nutrient Absorption-Related Genes and Intestinal Histology in Japanese Quails (Coturnix japonica) Throughout Their Productive Lifespan.","authors":"Hamid Reza Rafieian-Naeini, Mahdi Zhandi, Maryam Taghipour-Shahbandi, Amir Reza Sabzian, Ali Reza Yousefi, Mahboubeh Khajehnabi, Woo Kyun Kim","doi":"10.1002/vms3.70056","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Quail is an efficient poultry species known for many years, and its productivity is highly affected by gastrointestinal function, health, digestion and commensal bacteria. Specific histological parameters and nutrient absorption-related genes could be used to study intestinal functionality and efficiency.</p><p><strong>Objectives: </strong>This study aimed to evaluate the mRNA abundance of nutrient absorption-related genes and intestinal histology changes in laying Japanese quail during productive lifespan.</p><p><strong>Methods: </strong>A total of 135 quails from a commercial flock at different egg production periods (early production [EP, 6 weeks of age], pick production [PP, 21 weeks of age] and post pick production [PPP, 40 weeks of age]) were randomly selected (n = 45/group) and kept for 6 weeks. Production performance was evaluated during last 2 weeks of the experimental period, and tissue samples were taken from the duodenum, jejunum and ileum for histological analysis and mRNA abundance purposes at the end of the experiment. The mRNA abundance of the CALB1, SGLT1, SGLT5, EAAT3 and NaPi-IIb genes were compared among groups.</p><p><strong>Results: </strong>Production period did not affect the eggshell thickness, goblet cell number and mRNA abundance of CALB1, EAAT3 and NaPi-IIb (p > 0.05). The PPP quails had the highest egg weight and FCR compared to EP and PP quails (p < 0.001). Egg production and egg mass of the EP and PP quails were higher than those of PPP quails (p < 0.001). Moreover, EP quails had the highest villus height (VH)/crypt depth (CD) ratio in the duodenum and jejunum and the lowest VH:CD in the ileum compared with the PPP quails (p < 0.01). The lowest SGLT1 mRNA abundance was noted in the PP quails, whereas EP quails had the highest SGLT5 mRNA abundance (p < 0.05).</p><p><strong>Conclusions: </strong>In conclusion, although histology parameter changes were in-line with the lowest performance observed in the PPP quails, the current results failed to show a relationship between mRNA abundance changes and production performance attributes at different productive lifespans.</p>","PeriodicalId":23543,"journal":{"name":"Veterinary Medicine and Science","volume":"10 6","pages":"e70056"},"PeriodicalIF":1.8000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11519991/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Medicine and Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1002/vms3.70056","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Quail is an efficient poultry species known for many years, and its productivity is highly affected by gastrointestinal function, health, digestion and commensal bacteria. Specific histological parameters and nutrient absorption-related genes could be used to study intestinal functionality and efficiency.

Objectives: This study aimed to evaluate the mRNA abundance of nutrient absorption-related genes and intestinal histology changes in laying Japanese quail during productive lifespan.

Methods: A total of 135 quails from a commercial flock at different egg production periods (early production [EP, 6 weeks of age], pick production [PP, 21 weeks of age] and post pick production [PPP, 40 weeks of age]) were randomly selected (n = 45/group) and kept for 6 weeks. Production performance was evaluated during last 2 weeks of the experimental period, and tissue samples were taken from the duodenum, jejunum and ileum for histological analysis and mRNA abundance purposes at the end of the experiment. The mRNA abundance of the CALB1, SGLT1, SGLT5, EAAT3 and NaPi-IIb genes were compared among groups.

Results: Production period did not affect the eggshell thickness, goblet cell number and mRNA abundance of CALB1, EAAT3 and NaPi-IIb (p > 0.05). The PPP quails had the highest egg weight and FCR compared to EP and PP quails (p < 0.001). Egg production and egg mass of the EP and PP quails were higher than those of PPP quails (p < 0.001). Moreover, EP quails had the highest villus height (VH)/crypt depth (CD) ratio in the duodenum and jejunum and the lowest VH:CD in the ileum compared with the PPP quails (p < 0.01). The lowest SGLT1 mRNA abundance was noted in the PP quails, whereas EP quails had the highest SGLT5 mRNA abundance (p < 0.05).

Conclusions: In conclusion, although histology parameter changes were in-line with the lowest performance observed in the PPP quails, the current results failed to show a relationship between mRNA abundance changes and production performance attributes at different productive lifespans.

探究日本鹌鹑(Coturnix japonica)整个生育期与营养吸收相关的基因和肠道组织学。
背景:鹌鹑是一种高效家禽,其生产率受肠道功能、健康、消化和共生菌的影响很大。特定的组织学参数和营养吸收相关基因可用于研究肠道功能和效率:本研究旨在评估产蛋日本鹌鹑在产蛋期营养吸收相关基因的 mRNA 丰度和肠道组织学变化:随机选取135只处于不同产蛋期(早期产蛋期[EP,6周龄]、采摘期[PP,21周龄]和采摘后产蛋期[PPP,40周龄])的商品鹌鹑(n = 45/组),饲养6周。在实验的最后两周评估生产性能,并在实验结束时从十二指肠、空肠和回肠采集组织样本进行组织学分析和 mRNA 丰度分析。比较各组之间 CALB1、SGLT1、SGLT5、EAAT3 和 NaPi-IIb 基因的 mRNA 丰度:结果:生产期对蛋壳厚度、腺细胞数量以及 CALB1、EAAT3 和 NaPi-IIb 的 mRNA 丰度均无影响(p > 0.05)。与 EP 和 PP 型鹌鹑相比,PPP 型鹌鹑的蛋重和 FCR 最高(p 结论:PPP 型鹌鹑的蛋重和 FCR 均低于 EP 型鹌鹑:总之,虽然组织学参数变化与 PPP 鹌鹑的最低生产性能一致,但目前的结果未能显示 mRNA 丰度变化与不同生产寿命的生产性能属性之间的关系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Veterinary Medicine and Science
Veterinary Medicine and Science Veterinary-General Veterinary
CiteScore
3.00
自引率
0.00%
发文量
296
期刊介绍: Veterinary Medicine and Science is the peer-reviewed journal for rapid dissemination of research in all areas of veterinary medicine and science. The journal aims to serve the research community by providing a vehicle for authors wishing to publish interesting and high quality work in both fundamental and clinical veterinary medicine and science. Veterinary Medicine and Science publishes original research articles, systematic reviews, meta-analyses, and research methods papers, along with invited editorials and commentaries. Original research papers must report well-conducted research with conclusions supported by the data presented in the paper. We aim to be a truly global forum for high-quality research in veterinary medicine and science, and believe that the best research should be published and made widely accessible as quickly as possible. Veterinary Medicine and Science publishes papers submitted directly to the journal and those referred from a select group of prestigious journals published by Wiley-Blackwell. Veterinary Medicine and Science is a Wiley Open Access journal, one of a new series of peer-reviewed titles publishing quality research with speed and efficiency. For further information visit the Wiley Open Access website.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信