Multi-component resistance responses of melon to zucchini yellow mosaic virus.

IF 2.6 2区农林科学 Q2 PLANT SCIENCES

Phytopathology Pub Date : 2024-10-29 DOI:10.1094/PHYTO-05-24-0166-R

Líllian B J Bibiano, Camila P Carvalho, Ricardo Gioria, Jorge A M Rezende, Luis E A Camargo

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引用次数: 0

Abstract

A major resistance gene of the melon accession PI414723 to zucchini yellow mosaic virus (ZYMV) is located at the Zym-1 locus on chromosome 2, but the underlying defense mechanisms are poorly understood. The physiological responses and expression of selected genes at Zym-1 were assessed in PI414723 and in the susceptible genotype Védrantais. Viral titers and the expression of genes related to systemic acquired resistance (SAR) were evaluated in inoculated (Inoc) and non-inoculated (Non-Inoc) portions of the cotyledons at 3, 7 and 10 days after inoculation (dai) and in apical leaves at 10 dai. ZYMV was detected in both portions of the cotyledons but not in the apical leaves of PI414723 plants. Also, ZYMV was recovered in a susceptible zucchini only from Inoc portions at 3 dai. By contrast, in Védrantais ZYMV was detected and recovered from all tissues at high concentrations. Starchy local lesions and accumulation of transcripts of the SAR marker genes PR1 and PR4 were also detected in the resistant genotype. Plus, transcripts of one candidate resistance gene analog previously located at Zym-1 and of two melon homologs of restricted tobacco etch virus movement 2 (RTM2) genes located close to Zym-1, accumulated only in PI414723. It is proposed that resistance results from the combined action of the R gene, involved in restricting ZYMV replication after a supposed recognition event and of the RTM genes which impact viral systemic movement to distal apical tissues.

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甜瓜对西葫芦黄镶嵌病毒的多成分抗性反应。

甜瓜品种 PI414723 对西葫芦黄镶嵌病毒（ZYMV）的主要抗性基因位于 2 号染色体上的 Zym-1 基因座，但对其潜在的防御机制却知之甚少。我们评估了 PI414723 和易感基因型 Védrantais 对 Zym-1 基因的生理反应和表达情况。在接种后 3 天、7 天和 10 天，对接种（Inoc）和未接种（Non-Inoc）部分子叶的病毒滴度和系统获得性抗性（SAR）相关基因的表达进行了评估；在接种后 10 天，对顶端叶片的病毒滴度和系统获得性抗性（SAR）相关基因的表达进行了评估。在 PI414723 植株的子叶两部分都检测到了 ZYMV，但在顶端叶片中没有检测到。此外，在一个易感西葫芦中，只有在 3 dai 时才从 Inoc 部分中检测到 ZYMV。相比之下，在 Védrantais 的所有组织中都能检测到高浓度的 ZYMV。在抗性基因型中还检测到局部淀粉病变和 SAR 标记基因 PR1 和 PR4 的转录本积累。此外，以前位于 Zym-1 的一个候选抗性基因类似物和位于 Zym-1 附近的两个限制性烟草蚀变病毒移动 2（RTM2）基因的甜瓜同源物的转录物仅在 PI414723 中积累。据推测，抗性是 R 基因和 RTM 基因共同作用的结果，前者在假定的识别事件后参与限制 ZYMV 的复制，后者则影响病毒向远端顶端组织的系统移动。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phytopathology 生物-植物科学

CiteScore

5.90

自引率

9.40%

发文量

505

审稿时长

4-8 weeks

期刊介绍： Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.