Synergistic effect of antibiotics, α-linolenic acid and solvent type against Staphylococcus aureus biofilm formation.

IF 3.6 3区医学 Q2 PHARMACOLOGY & PHARMACY

Pharmacological Reports Pub Date : 2024-12-01 Epub Date: 2024-10-28 DOI:10.1007/s43440-024-00669-3

Karolina Knap, Konrad Kwiecień, Dorota Ochońska, Katarzyna Reczyńska-Kolman, Elżbieta Pamuła, Monika Brzychczy-Włoch

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引用次数: 0

Abstract

Background: A promising approach to the treatment of bacterial infections involves inhibiting the quorum sensing (QS) mechanism to prevent the formation and growth of bacterial biofilm. While antibiotics are used to kill remaining bacteria, QS inhibitors (QSIs) allow for antibiotic doses to be reduced. This study focuses on evaluating the synergy between gentamicin sulphate (GEN), tobramycin (TOB), or azithromycin (AZM) with linolenic acid (LNA) against the formation of an early Staphylococcus aureus biofilm.

Methods: Minimum biofilm inhibitory concentration (MBIC) was determined using the resazurin reduction assay for all antibiotics and LNA. The reduction of biofilm mass was assessed using the crystal violet (CV) assay. We have also evaluated the effect of dimethyl sulfoxide with TWEEN (DMSO_T) on early biofilm formation. Synergy was determined by metabolic activity assay and fractional biofilm inhibitory concentration (FBIC).

Results: DMSO_T at a concentration of 1% enhanced early biofilm formation, but also decreased the doses of antibiotic needed to reduce the biofilm by up to 8 times. Adding LNA at a concentration of 32 µg/ml or 64 µg/ml allowed up to a 32-fold reduction of antibiotic doses for GEN and TOB and a 4-fold reduction for AZM.

Conclusions: LNA's use in combination with various antibiotics could reduce their doses and help fight drug-resistant bacteria in the biofilm.

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抗生素、α-亚麻酸和溶剂类型对金黄色葡萄球菌生物膜形成的协同效应

背景：治疗细菌感染的一种可行方法是抑制法定量感应（QS）机制，防止细菌生物膜的形成和生长。虽然抗生素可用于杀死残留细菌，但 QS 抑制剂（QSIs）可减少抗生素剂量。本研究主要评估硫酸庆大霉素（GEN）、妥布霉素（TOB）或阿奇霉素（AZM）与亚麻酸（LNA）对早期金黄色葡萄球菌生物膜形成的协同作用：所有抗生素和 LNA 的最小生物膜抑制浓度（MBIC）都是用雷沙唑啉还原法测定的。使用水晶紫（CV）检测法评估了生物膜质量的减少情况。我们还评估了二甲基亚砜与 TWEEN（DMSO_T）对早期生物膜形成的影响。协同作用通过代谢活性测定和生物膜抑制浓度分数（FBIC）来确定：结果：浓度为 1%的 DMSO_T 可促进早期生物膜的形成，同时还可将减少生物膜所需的抗生素剂量减少多达 8 倍。加入浓度为 32 微克/毫升或 64 微克/毫升的 LNA 可使 GEN 和 TOB 的抗生素剂量减少 32 倍，使 AZM 的抗生素剂量减少 4 倍：结论：将 LNA 与各种抗生素结合使用可减少抗生素的剂量，有助于抗击生物膜中的耐药菌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmacological Reports 医学-药学

CiteScore

8.40

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Pharmacological Reports publishes articles concerning all aspects of pharmacology, dealing with the action of drugs at a cellular and molecular level, and papers on the relationship between molecular structure and biological activity as well as reports on compounds with well-defined chemical structures. Pharmacological Reports is an open forum to disseminate recent developments in: pharmacology, behavioural brain research, evidence-based complementary biochemical pharmacology, medicinal chemistry and biochemistry, drug discovery, neuro-psychopharmacology and biological psychiatry, neuroscience and neuropharmacology, cellular and molecular neuroscience, molecular biology, cell biology, toxicology. Studies of plant extracts are not suitable for Pharmacological Reports.