Microfluidics-based stable production of monodisperse giant unilamellar vesicles by oil-phase removal from double emulsion.

IF 3.6 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Tomoki Yamada, Hiroaki Suzuki
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引用次数: 0

Abstract

Giant liposomes, or giant unilamellar vesicles (GUVs), have been utilized as cell-size bioreactors to replicate the physical and chemical properties of biological cells. However, conventional methods for preparing GUVs typically lack precise control over their size. Several research groups have recently developed microfluidic techniques to create monodisperse GUVs by generating water-in-oil-in-water (W/O/W) droplets with a thin oil layer that subsequently transform into GUVs. However, the formation of a thin oil shell requires the intricate control of the flow rate, which can be both challenging and unstable. In this study, we investigated the design of a two-step flow-focusing microfluidic channel to produce stable W/O/W droplets. These droplets underwent substantial oil layer reduction through spontaneous removal by fluidic shear forces. Consequently, the majority of the oil layer in the W/O/W droplets was reduced, improving uniformity of GUVs.

基于微流控技术,通过从双乳液中去除油相,稳定生产单分散巨型单酰胺囊泡。
巨型脂质体或巨型单酰胺囊泡 (GUV) 已被用作细胞大小的生物反应器,以复制生物细胞的物理和化学特性。然而,制备 GUVs 的传统方法通常缺乏对其大小的精确控制。一些研究小组最近开发了微流体技术,通过生成带有薄油层的水包油水型(W/O/W)液滴来制造单分散 GUV,随后将其转化为 GUV。然而,薄油壳的形成需要对流速进行复杂的控制,这既具有挑战性又不稳定。在本研究中,我们研究了如何设计一个两步流聚焦微流体通道,以产生稳定的 W/O/W 液滴。这些液滴在流体剪切力的作用下,油层自发脱落,从而大大减少了油层。因此,W/O/W 液滴中的大部分油层都减少了,从而提高了 GUV 的均匀性。
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来源期刊
Journal of Liposome Research
Journal of Liposome Research 生物-生化与分子生物学
CiteScore
10.50
自引率
2.30%
发文量
24
审稿时长
3 months
期刊介绍: The Journal of Liposome Research aims to publish original, high-quality, peer-reviewed research on the topic of liposomes and related systems, lipid-based delivery systems, lipid biology, and both synthetic and physical lipid chemistry. Reviews and commentaries or editorials are generally solicited and are editorially reviewed. The Journal also publishes abstracts and conference proceedings including those from the International Liposome Society. The scope of the Journal includes: Formulation and characterisation of systems Formulation engineering of systems Synthetic and physical lipid chemistry Lipid Biology Biomembranes Vaccines Emerging technologies and systems related to liposomes and vesicle type systems Developmental methodologies and new analytical techniques pertaining to the general area Pharmacokinetics, pharmacodynamics and biodistribution of systems Clinical applications. The Journal also publishes Special Issues focusing on particular topics and themes within the general scope of the Journal.
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