Melissa officinalis Regulates Lipopolysaccharide-Induced BV2 Microglial Activation via MAPK and Nrf2 Signaling.

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Ji-Won Choi, Sang Yoon Choi, Guijae Yoo, Ho-Young Park, In-Wook Choi, Jinyoung Hur
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引用次数: 0

Abstract

Neuroinflammation and microglial activation play critical roles in neurodegenerative diseases such as Alzheimer's and Parkinson's disease. Modulating microglial activation may help prevent the progression of these disorders. This study aimed to investigate the effects and mechanisms of Melissa officinalis ethanol extract on lipopolysaccharide (LPS)-induced microglial activation in BV2 cells. Cell viability and nitric oxide (NO) production were assessed using MTT assay and Griess reagent, while inflammatory cytokine levels were measured by qPCR. Key inflammatory pathways, including MAPK, TLR4, and antioxidant biomarkers, were analyzed through western blot and immunofluorescence. Rosmarinic acid content in M. officinalis was determined using high-performance liquid chromatography (HPLC). The results demonstrated that M. officinalis ethanol extract significantly inhibited LPS-induced NO production and reduced inflammatory cytokine expression. Additionally, it downregulated inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), TLR4, NF-κB, and MAPK signaling pathways (p38, JNK, ERK), while increasing the expression of antioxidant markers, including Nrf2, HO-1, catalase, and SOD2. In conclusion, M. officinalis ethanol extract exerts neuroprotective effects by modulating inflammation and enhancing antioxidant defenses, suggesting its potential in the prevention and treatment of inflammation-related neurodegenerative diseases.

Melissa officinalis 通过 MAPK 和 Nrf2 信号调节脂多糖诱导的 BV2 微神经胶质细胞活化
神经炎症和小胶质细胞活化在阿尔茨海默病和帕金森病等神经退行性疾病中起着至关重要的作用。调节小胶质细胞的活化可能有助于预防这些疾病的恶化。本研究旨在探讨香蜂草乙醇提取物对脂多糖(LPS)诱导的 BV2 细胞小胶质细胞活化的影响和机制。细胞活力和一氧化氮(NO)的产生采用 MTT 法和 Griess 试剂进行评估,炎症细胞因子水平采用 qPCR 法进行测定。通过 Western 印迹和免疫荧光分析了关键的炎症通路,包括 MAPK、TLR4 和抗氧化生物标志物。采用高效液相色谱法(HPLC)测定了迷迭香中的迷迭香酸含量。结果表明,M. officinalis 乙醇提取物能显著抑制 LPS 诱导的 NO 生成,减少炎症细胞因子的表达。此外,它还能下调诱导型一氧化氮合酶(iNOS)、环氧化酶-2(COX-2)、TLR4、NF-κB 和 MAPK 信号通路(p38、JNK、ERK),同时增加抗氧化标志物的表达,包括 Nrf2、HO-1、过氧化氢酶和 SOD2。总之,M. officinalis乙醇提取物通过调节炎症和增强抗氧化防御功能发挥神经保护作用,这表明它在预防和治疗与炎症相关的神经退行性疾病方面具有潜力。
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来源期刊
Journal of microbiology and biotechnology
Journal of microbiology and biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
5.50
自引率
3.60%
发文量
151
审稿时长
2 months
期刊介绍: The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
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