Bone sialoprotein facilitates anoikis resistance in lung cancer by inhibiting miR-150-5p expression

IF 5.3
Le Huynh Hoai Thuong, Chang-Lun Huang, Yi-Chin Fong, Chun-Lin Liu, Jeng-Hung Guo, Chih-Ying Wu, Po-I Liu, Chih-Hsin Tang
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Abstract

Metastatic lung cancer is a highly prevalent cancer with a very low chance of long-term survival. Metastasis at secondary sites requires that cancer cells develop anoikis resistance to survive during circulation. High levels of bone sialoprotein (BSP), a member of the small integrin-binding ligand N-linked glycoproteins (SIBLINGs), have been shown to promote the spread of lung cancer cells; however, the effects of BSP in anoikis resistance are largely unknown. In this study, we determined that BSP promotes anoikis resistance in lung cancer cells. BSP was also shown to promote the expression of E-cadherin and vimentin (epithelial-to-mesenchymal transition markers, which have been utilized as indicators of anoikis resistance). It appears that BSP facilitates MMP-14-dependent anoikis resistance by inhibiting the synthesis of miR-150-5p and activating the ERK signalling pathway. Knockdown of BSP expression was shown to block lung cancer metastasis by lowering anoikis resistance in vivo. These results indicate that BSP is a promising target to deal with anoikis resistance and metastasis in human lung cancers.

Abstract Image

骨硅蛋白通过抑制 miR-150-5p 的表达促进肺癌的抗肿瘤能力
转移性肺癌是一种高发癌症,长期存活的几率非常低。继发部位的转移需要癌细胞在循环过程中发展出抗免疫力才能存活。高水平的骨硅蛋白(BSP)是小整合素结合配体N-连接糖蛋白(SIBLINGs)的成员之一,已被证明能促进肺癌细胞的扩散;然而,BSP在抗免疫反应中的作用在很大程度上是未知的。在这项研究中,我们确定 BSP 能促进肺癌细胞的耐药力。研究还表明,BSP 还能促进 E-cadherin 和 vimentin(上皮细胞向间质转化的标志物,已被用作抗 anoikis 的指标)的表达。由此看来,BSP 通过抑制 miR-150-5p 的合成和激活 ERK 信号通路,促进了 MMP-14 依赖性厌氧菌抗性。研究表明,在体内敲除 BSP 的表达可通过降低耐 anoikis 性来阻止肺癌转移。这些结果表明,BSP 是解决人类肺癌耐 anoikis 性和转移问题的一个有前途的靶点。
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来源期刊
CiteScore
11.50
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期刊介绍: The Journal of Cellular and Molecular Medicine serves as a bridge between physiology and cellular medicine, as well as molecular biology and molecular therapeutics. With a 20-year history, the journal adopts an interdisciplinary approach to showcase innovative discoveries. It publishes research aimed at advancing the collective understanding of the cellular and molecular mechanisms underlying diseases. The journal emphasizes translational studies that translate this knowledge into therapeutic strategies. Being fully open access, the journal is accessible to all readers.
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