cba-miR-222-3p involved in photoperiod-induced apoptosis in testes of striped hamsters by targeting TRAF7.

IF 3.5 1区 生物学 Q1 ZOOLOGY
Shuo Wang, Jinhui Xu, Xingchen Wang, Mingdi Wang, Huiliang Xue, Ming Wu, Chao Fan, Lei Chen, Laixiang Xu
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Abstract

The role of miRNAs in the regulation of seasonal reproduction in rodents, particularly in relation to photoperiod changes, is still poorly understood. Previous studies on miRNA transcriptomes of striped hamster (Cricetulus barabensis) testes have indicated that the photoperiodism of testes, especially apoptosis, may be influenced by miRNAs. As a functional miRNA, cba-miR-222-3p in striped hamster testes exhibits suppression under a short photoperiod. To elucidate the potential role of testicular cba-miR-222-3p in the seasonal reproduction of striped hamsters, we exposed male striped hamsters to different photoperiods or injected miRNA agomir into the testes and observed the effects of these treatments, particularly some indicators related to apoptosis. The results showed that the levels of apoptosis in the testes increased in short daylength, accompanied by a significant decrease in cba-miR-222-3p expression and an increase in TRAF7 expression. Dual luciferase reporter assays verified the targeting relationship between cba-miR-222-3p and TRAF7 predicted by bioinformatics. In addition, the expression of TRAF7 decreased in the testes, which injected miRNA agomir, leading to inhibition of apoptosis, and the expression of key genes (MEKK3, p38, p53) in the downstream MAPK signaling pathway of TRAF7 was suppressed. These results suggest that short daylength induces testicular apoptosis in striped hamsters, and one possible mechanism is that the decreased expression of miR-222-3p in testes reduces the repression of TRAF7 translation, thereby activating the MAPK pathway and affecting the level of testicular apoptosis. These findings reveal the potential role of miR-222-3p in animal reproduction and provide new insights into the regulation of rodent populations.

cba-miR-222-3p 通过靶向 TRAF7 参与光周期诱导的条纹仓鼠睾丸凋亡。
人们对miRNA在啮齿类动物季节性繁殖调控中的作用,特别是与光周期变化的关系还知之甚少。以前对条纹仓鼠(Cricetulus barabensis)睾丸的 miRNA 转录组的研究表明,睾丸的光周期变化,特别是凋亡,可能受 miRNA 的影响。作为一种功能性 miRNA,条纹仓鼠睾丸中的 cba-miR-222-3p 在短光周期下表现出抑制作用。为了阐明睾丸cba-miR-222-3p在条纹仓鼠季节性繁殖中的潜在作用,我们将雄性条纹仓鼠暴露于不同的光周期或向睾丸注射miRNA agomir,并观察了这些处理的影响,特别是一些与细胞凋亡相关的指标。结果表明,睾丸中的细胞凋亡水平在短日照下增加,同时cba-miR-222-3p的表达显著下降,TRAF7的表达增加。双荧光素酶报告实验验证了生物信息学预测的 cba-miR-222-3p 和 TRAF7 之间的靶向关系。此外,注射了 miRNA agomir 的睾丸中 TRAF7 的表达量减少,导致凋亡受到抑制,TRAF7 下游 MAPK 信号通路中的关键基因(MEKK3、p38、p53)的表达受到抑制。这些结果表明,短日照会诱导条纹仓鼠睾丸凋亡,其中一个可能的机制是 miR-222-3p 在睾丸中的表达量减少,降低了对 TRAF7 翻译的抑制,从而激活了 MAPK 通路,影响了睾丸凋亡的水平。这些发现揭示了 miR-222-3p 在动物繁殖中的潜在作用,并为啮齿动物种群的调控提供了新的见解。
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来源期刊
CiteScore
6.40
自引率
12.10%
发文量
81
审稿时长
>12 weeks
期刊介绍: The official journal of the International Society of Zoological Sciences focuses on zoology as an integrative discipline encompassing all aspects of animal life. It presents a broader perspective of many levels of zoological inquiry, both spatial and temporal, and encourages cooperation between zoology and other disciplines including, but not limited to, physics, computer science, social science, ethics, teaching, paleontology, molecular biology, physiology, behavior, ecology and the built environment. It also looks at the animal-human interaction through exploring animal-plant interactions, microbe/pathogen effects and global changes on the environment and human society. Integrative topics of greatest interest to INZ include: (1) Animals & climate change (2) Animals & pollution (3) Animals & infectious diseases (4) Animals & biological invasions (5) Animal-plant interactions (6) Zoogeography & paleontology (7) Neurons, genes & behavior (8) Molecular ecology & evolution (9) Physiological adaptations
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