Genetic characterization of Neisseria meningitidis isolates recovered from patients with invasive meningococcal disease in Lithuania.

IF 4.6 2区 医学 Q2 IMMUNOLOGY
Frontiers in Cellular and Infection Microbiology Pub Date : 2024-10-14 eCollection Date: 2024-01-01 DOI:10.3389/fcimb.2024.1432197
Anželika Slavinska, Magdalena Kowalczyk, Agnė Kirkliauskienė, Greta Vizuje, Paweł Siedlecki, Joana Bikulčienė, Kristina Tamošiūnienė, Aurelija Petrutienė, Nomeda Kuisiene
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引用次数: 0

Abstract

Introduction: Neisseria meningitidis is a gram-negative bacterium responsible for life-threatening invasive infections known as invasive meningococcal disease and is associated with high fatality rates and serious lifelong disabilities among survivors.

Methods: This study aimed to characterize N. meningitidis isolates cultured from blood and cerebrospinal fluid collected between 2009 and 2021 in Lithuania, assess their genomic relationships with European strains, and evaluate the possibility of using a cost-effective method for strain characterization, thus improving the national molecular surveillance of invasive meningococcal disease. In total, 321 N. meningitidis isolates were collected and analyzed using multilocus restriction typing (MLRT). Amplification of the penA gene and restriction fragment length polymorphism analysis were performed to identify the modified penA genes. Based on the MLRT genotyping results, we selected 10 strains for additional analysis using whole-genome sequencing. The sequenced genomes were incorporated into a dataset of publicly available N. meningitidis genomes to evaluate genomic diversity and establish phylogenetic relationships within the Lithuanian and European circulating strains.

Results: We identified 83 different strains using MLRT genotyping. Genomic diversity of N. meningitidis genomes analysed revealed 21 different sequence types (STs) circulating in Lithuania. Among these, ST34 was the most prevalent. Notably, three isolates displayed unique combinations of seven housekeeping genes and were identified as novel STs: ST16969, ST16901, and ST16959. The analyzed strains were found to possess virulence factors not commonly found in N. meningitidis. Six distinct penA profiles were identified, each with different frequencies. In the present study, we also identified N. meningitidis strains with new penA, NEIS0123, NEIS1320, NEIS1525, NEIS1600, and NEIS1753 loci variants. In our study, using the cgMLST scheme, Minimum Spanning Tree (MST) analysis did not identify significant geographic relationships between Lithuanian N. meningitidis isolates and strains from Europe.

Discussion: Discussion: To our knowledge, this is the first study to employ whole genome sequencing (WGS) method for a comprehensive genetic characterization of invasive N. meningitidis isolates from Lithuania. This approach provides a more detailed and precise analysis of genomic relationships and diversity compared to prior studies relying on traditional molecular typing methods and antigen analysis.

从立陶宛侵袭性脑膜炎球菌疾病患者中分离出的脑膜炎奈瑟菌的基因特征。
导言:脑膜炎奈瑟菌是一种革兰氏阴性菌,可导致危及生命的侵袭性感染,即侵袭性脑膜炎球菌病,其致死率很高,幸存者会终身严重残疾:本研究旨在描述 2009 年至 2021 年期间从立陶宛收集的血液和脑脊液中培养出的脑膜炎球菌分离物的特征,评估它们与欧洲菌株的基因组关系,并评估使用具有成本效益的方法进行菌株特征描述的可能性,从而改进对侵袭性脑膜炎球菌病的国家分子监测。共收集了 321 株脑膜炎球菌分离株,并使用多焦点限制性分型(MLRT)进行了分析。通过扩增 penA 基因和限制性片段长度多态性分析,确定了变异的 penA 基因。根据 MLRT 基因分型结果,我们选择了 10 株菌株进行全基因组测序。测序后的基因组被纳入公开的脑膜炎双球菌基因组数据集,以评估立陶宛和欧洲流行菌株的基因组多样性并建立系统发育关系:结果:我们利用 MLRT 基因分型鉴定出了 83 个不同的菌株。分析脑膜炎球菌基因组的基因组多样性发现,在立陶宛流行的脑膜炎球菌有 21 种不同的序列类型(ST)。其中,ST34最为普遍。值得注意的是,有三个分离株显示出七个看家基因的独特组合,并被鉴定为新型 ST:ST16969、ST16901 和 ST16959。分析发现,这些菌株具有脑膜炎奈瑟菌中不常见的毒力因子。我们发现了六种不同的 penA 特征,每种特征都有不同的频率。在本研究中,我们还发现了具有新的 penA、NEIS0123、NEIS1320、NEIS1525、NEIS1600 和 NEIS1753 位点变异的脑膜炎球菌菌株。在我们的研究中,使用 cgMLST 方案,最小生成树(MST)分析并未发现立陶宛脑膜炎球菌分离株与欧洲菌株之间存在显著的地理关系:讨论:讨论:据我们所知,这是首次采用全基因组测序(WGS)方法对来自立陶宛的入侵脑膜炎球菌分离株进行全面遗传特征描述的研究。与之前依赖传统分子分型方法和抗原分析的研究相比,这种方法能更详细、更精确地分析基因组关系和多样性。
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来源期刊
CiteScore
7.90
自引率
7.00%
发文量
1817
审稿时长
14 weeks
期刊介绍: Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.
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