Systematic review and meta-analysis of antigen rapid diagnostic tests to detect Zaire ebolavirus

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2024-10-20 DOI:10.1016/j.diagmicrobio.2024.116568

Devy M. Emperador , Cassandra Kelly-Cirino , Daniel G. Bausch , Isabella Eckerle

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Abstract

We conducted a systematic review and meta-analysis of studies and reports comparing the performance of antigen rapid diagnostic tests (Ag RDT) for diagnosing Ebola disease (EVD). We searched PubMed, EMBASE, and Web of Science for diagnostic studies published between 1976 and 2023, evaluating them with QUADAS-2. Using a bivariate random-effects model, we estimated the pooled sensitivity and specificity of Ag RDTs. Of 64 eligible full studies and reports, 16 met the inclusion criteria. Pooled sensitivity and specificity were 82.1% (95%CI: 75.2 – 88.0) and 97.0% (95%CI: 95.1-98.2), respectively. We conducted subgroup analysis on 4 Ag RDTs, 3 RT-PCR tests, and 4 sample types, showing varied performance. The high specificity and positive predictive value of Ag RDTs support their use to “rule-in” patients with EVD. However, high-sensitivity RDTs suitable for field settings and capable of detecting multiple ebolavirus species are needed.

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检测扎伊尔伊波拉病毒的抗原快速诊断测试的系统回顾和荟萃分析。

我们对比较抗原快速诊断测试（Ag RDT）诊断埃博拉疾病（EVD）性能的研究和报告进行了系统回顾和荟萃分析。我们在 PubMed、EMBASE 和 Web of Science 上检索了 1976 年至 2023 年间发表的诊断研究，并使用 QUADAS-2 对这些研究进行了评估。利用双变量随机效应模型，我们估算了Ag RDT的总体灵敏度和特异性。在 64 项符合条件的完整研究和报告中，有 16 项符合纳入标准。汇总灵敏度和特异性分别为 82.1%（95%CI：75.2 - 88.0）和 97.0%（95%CI：95.1-98.2）。我们对 4 种 Ag RDT、3 种 RT-PCR 检测和 4 种样本类型进行了亚组分析，结果显示出不同的性能。Ag RDT 的高特异性和阳性预测值支持将其用于 "排除 "EVD 患者。不过，还需要适合现场环境并能检测多种埃博拉病毒的高灵敏度 RDT。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.