Germ cell progression through zebrafish spermatogenesis declines with age.

IF 3.7 2区生物学 Q1 DEVELOPMENTAL BIOLOGY

Development Pub Date : 2024-10-29 DOI:10.1242/dev.204319

Andrea L Sposato, Hailey L Hollins, Darren R Llewellyn, Jenna M Weber, Madison N Schrock, Jeffrey A Farrell, James A Gagnon

引用次数: 0

Abstract

Vertebrate spermatogonial stem cells maintain sperm production over the lifetime of an animal but fertility declines with age. While morphological studies have informed our understanding of typical spermatogenesis, the molecular and cellular mechanisms underlying the maintenance and decline of spermatogenesis are not yet understood. We used single-cell RNA sequencing to generate a developmental atlas of the aging zebrafish testis. All testes contained spermatogonia, but we observed a progressive decline in spermatogenesis that correlates with age. Testes from some older males only contained spermatogonia and a reduced population of spermatocytes. Spermatogonia in older males are transcriptionally distinct from spermatogonia in testes capable of robust spermatogenesis. Immune cells including macrophages and lymphocytes drastically increase in abundance in testes that cannot complete spermatogenesis. Our developmental atlas reveals the cellular changes as the testis ages and defines a molecular roadmap for the regulation of spermatogenesis.

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斑马鱼精子发生过程中生殖细胞的发育随年龄增长而衰退。

脊椎动物的精原干细胞能在动物的一生中维持精子生成，但生育能力会随着年龄的增长而下降。虽然形态学研究有助于我们了解典型的精子发生，但精子发生的维持和衰退的分子和细胞机制尚不清楚。我们利用单细胞 RNA 测序生成了衰老斑马鱼睾丸的发育图谱。所有睾丸都含有精原细胞，但我们观察到精子发生随年龄的增长而逐渐衰退。一些年龄较大的雄性斑马鱼睾丸只含有精原细胞，精母细胞数量减少。老年男性睾丸中的精原细胞在转录上有别于精子发生旺盛的睾丸中的精原细胞。在无法完成精子发生的睾丸中，包括巨噬细胞和淋巴细胞在内的免疫细胞数量急剧增加。我们的发育图谱揭示了睾丸衰老过程中的细胞变化，并定义了精子发生调控的分子路线图。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Development 生物-发育生物学

CiteScore

6.70

自引率

4.30%

发文量

433

审稿时长

3 months

期刊介绍： Development’s scope covers all aspects of plant and animal development, including stem cell biology and regeneration. The single most important criterion for acceptance in Development is scientific excellence. Research papers (articles and reports) should therefore pose and test a significant hypothesis or address a significant question, and should provide novel perspectives that advance our understanding of development. We also encourage submission of papers that use computational methods or mathematical models to obtain significant new insights into developmental biology topics. Manuscripts that are descriptive in nature will be considered only when they lay important groundwork for a field and/or provide novel resources for understanding developmental processes of broad interest to the community. Development includes a Techniques and Resources section for the publication of new methods, datasets, and other types of resources. Papers describing new techniques should include a proof-of-principle demonstration that the technique is valuable to the developmental biology community; they need not include in-depth follow-up analysis. The technique must be described in sufficient detail to be easily replicated by other investigators. Development will also consider protocol-type papers of exceptional interest to the community. We welcome submission of Resource papers, for example those reporting new databases, systems-level datasets, or genetic resources of major value to the developmental biology community. For all papers, the data or resource described must be made available to the community with minimal restrictions upon publication. To aid navigability, Development has dedicated sections of the journal to stem cells & regeneration and to human development. The criteria for acceptance into these sections is identical to those outlined above. Authors and editors are encouraged to nominate appropriate manuscripts for inclusion in one of these sections.