Carboxyl hybrid monolithic column in-tube solid-phase microextraction coupled with UPLC-QTRAP MS/MS for the determination of amphetamine-type stimulants

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
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引用次数: 0

Abstract

A carboxyl functionalized organic-inorganic hybrid monolithic column (TMOS-co-CES) was applied as in-tube solid-phase microextraction (SPME) sorbent combining with ultra-performance liquid chromatography-triple quadrupole/linear ion trap mass spectrometer for separation and analyzation of seven typical amphetamine-type stimulants (ATSs), including amphetamine (AM), methamphetamine (MAM), cathinone, methcathinone, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine and 3,4-methylenedioxyethylamphetamine. The application potential of TMOS-co-CES material to ATSs was preliminarily confirmed by computational simulation by using cathinone as a representative. The influences of various SPME parameters and analytical performance were investigated systematically. As matched with the results of computational simulation, TMOS-co-CES column could capture ATSs under milder near neutral pH condition with high extraction efficiency basing on the adsorption mechanism explained as a mixed mode of electrostatic and hydrophobic interactions. Seven target trace ATSs in spiked sewage, pond water and urine could be rapidly and conveniently separated and enriched by the proposed TMOS-co-CES in-tube SPME method under the optimized conditions with good accuracy, repeatability and resistance to matrix interference. Moreover, AM and MAM had been successfully detected in real urines of suspected drug abusers by TMOS-co-CES in-tube SPME method, which indicated that the proposed method had good application feasibility for drug monitoring. The mild extraction condition and ideal method performance further made the TMOS-co-CES in-tube SPME method more potential in applications for forensic analysis and drug abuse.
羧基杂化整体柱管内固相微萃取与 UPLC-QTRAP MS/MS 联用测定苯丙胺类兴奋剂。
应用羧基功能化有机-无机杂化整体柱(TMOS-co-CES)作为管内固相微萃取(SPME)吸附剂,结合超高效液相色谱-三重四极杆/线性离子阱质谱仪,分离分析了七种典型的苯丙胺类兴奋剂(ATSs)、包括苯丙胺(AM)、甲基苯丙胺(MAM)、卡西酮、甲卡西酮、3,4-亚甲二氧基苯丙胺、3,4-亚甲二氧基甲基苯丙胺和 3,4-亚甲二氧基乙基苯丙胺。以卡西酮为代表,通过计算模拟初步证实了 TMOS-co-CES 材料在苯丙胺类兴奋剂中的应用潜力。系统研究了各种 SPME 参数对分析性能的影响。与计算模拟结果相一致,TMOS-co-CES 柱在较温和的近中性 pH 条件下,基于静电和疏水相互作用混合模式的吸附机理,能以较高的萃取效率捕获苯丙胺类兴奋剂。在优化的条件下,采用TMOS-co-CES管内SPME方法可以快速、方便地分离和富集污水、池塘水和尿液中的7种目标痕量苯并咪唑类兴奋剂,具有良好的准确性、重复性和抗基体干扰性。此外,TMOS-co-CES管内SPME法成功地检测了吸毒嫌疑人真实尿液中的AM和MAM,表明该方法在毒品监测中具有良好的应用前景。TMOS-co-CES试管内SPME法的萃取条件温和、方法性能理想,使其在法医分析和药物滥用方面的应用更具潜力。
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来源期刊
Journal of Chromatography A
Journal of Chromatography A 化学-分析化学
CiteScore
7.90
自引率
14.60%
发文量
742
审稿时长
45 days
期刊介绍: The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.
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