Response of nitrifier and denitrifier community to Epichloë endophytes mediated host litter decomposition under phosphorus addition treatments

IF 3.9 2区农林科学 Q1 AGRONOMY

Plant and Soil Pub Date : 2024-10-28 DOI:10.1007/s11104-024-07019-5

Jie Jin, Chao Wang, Yang Yang, Ronggui Liu, Rong Zheng, Maohua Deng, Jianfeng Wang

{"title":"Response of nitrifier and denitrifier community to Epichloë endophytes mediated host litter decomposition under phosphorus addition treatments","authors":"Jie Jin, Chao Wang, Yang Yang, Ronggui Liu, Rong Zheng, Maohua Deng, Jianfeng Wang","doi":"10.1007/s11104-024-07019-5","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Background and aim</h3>Nutrient availability plays a crucial role in both litter decomposition and decomposer communities. However, the specific effects of Epichloë endophytes on soil decomposer communities during the decomposition of their host litter under fertilized conditions are still unclear.<h3 data-test=\"abstract-sub-heading\">Methods</h3>In this study, qPCR combined with Illumina sequencing was conducted to reveal the responses of functional gene communities involved in organic P recycling (phoD), nitrification (AOA/AOB-amoA), and denitrification (nirS/nirK) to Epichloë gansuensis-mediated decomposition of Achnatherum inebrians litter under different P addition treatments. Meanwhile, soil physicochemical properties and enzyme activities were measured.<h3 data-test=\"abstract-sub-heading\">Results</h3>Both E. gansuensis infection and P addition exerted limited effect on the abundance and diversity of phoD-harboring microbial community. In the E. gansuensis-free (E−) samples, 60 mM P addition significantly increased the gene copies of AOA-amoA and significantly decreased those of AOB-amoA. Additionally, P addition decreased the richness and diversity of the nirK-harboring community in the E− samples, while E. gansuensis appeared to alleviate this response. P addition decreased the relative abundance of nirS-harboring Acidovorax and Azospirillum, while increasing the nirK-harboring Rhodopseudomonas and Bosea. Mantel’s test showed that E. gansuensis infection decreased the correlation between nitrifier communities and soil properties. Furthermore, nirS- and nirK-harboring communities showed a similar correlation pattern with soil properties in both E+ and E− soil samples.<h3 data-test=\"abstract-sub-heading\">Conclusions</h3>This study demonstrates the important roles of E. gansuensis on nitrifier and denitrifier communities during its host grass decomposition under different P nutrient conditions, facilitating our comprehensive understanding of the ecological significance of Epichloë endophytes.","PeriodicalId":20223,"journal":{"name":"Plant and Soil","volume":"15 1","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant and Soil","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11104-024-07019-5","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

Background and aim

Nutrient availability plays a crucial role in both litter decomposition and decomposer communities. However, the specific effects of Epichloë endophytes on soil decomposer communities during the decomposition of their host litter under fertilized conditions are still unclear.

Methods

In this study, qPCR combined with Illumina sequencing was conducted to reveal the responses of functional gene communities involved in organic P recycling (phoD), nitrification (AOA/AOB-amoA), and denitrification (nirS/nirK) to Epichloë gansuensis-mediated decomposition of Achnatherum inebrians litter under different P addition treatments. Meanwhile, soil physicochemical properties and enzyme activities were measured.

Results

Both E. gansuensis infection and P addition exerted limited effect on the abundance and diversity of phoD-harboring microbial community. In the E. gansuensis-free (E−) samples, 60 mM P addition significantly increased the gene copies of AOA-amoA and significantly decreased those of AOB-amoA. Additionally, P addition decreased the richness and diversity of the nirK-harboring community in the E− samples, while E. gansuensis appeared to alleviate this response. P addition decreased the relative abundance of nirS-harboring Acidovorax and Azospirillum, while increasing the nirK-harboring Rhodopseudomonas and Bosea. Mantel’s test showed that E. gansuensis infection decreased the correlation between nitrifier communities and soil properties. Furthermore, nirS- and nirK-harboring communities showed a similar correlation pattern with soil properties in both E+ and E− soil samples.

Conclusions

This study demonstrates the important roles of E. gansuensis on nitrifier and denitrifier communities during its host grass decomposition under different P nutrient conditions, facilitating our comprehensive understanding of the ecological significance of Epichloë endophytes.

Abstract Image

查看原文本刊更多论文

加磷处理下硝化细菌和反硝化细菌群落对 Epichloë 内生菌介导的宿主垃圾分解的响应

背景和目的营养物质的可获得性在废弃物分解和分解者群落中都起着至关重要的作用。然而，在施肥条件下，Epichloë内生菌分解寄主枯落物时对土壤分解者群落的具体影响仍不清楚。方法本研究采用qPCR结合Illumina测序技术，揭示了在不同的P添加处理下，参与有机P循环（phoD）、硝化（AOA/AOB-amoA）和反硝化（nirS/nirK）的功能基因群落对Epichloë gansuensis介导的Achnatherum inebrians枯落物分解的响应。结果E. gansuensis感染和P添加对phoD-harboring微生物群落的丰度和多样性影响有限。在不含 E. gansuensis（E-）的样品中，添加 60 mM P 会显著增加 AOA-amoA 的基因拷贝数，而显著减少 AOB-amoA 的基因拷贝数。此外，P 的添加降低了 E- 样本中 nirK-arboring 群落的丰富度和多样性，而 E. gansuensis 似乎减轻了这种反应。P 的添加降低了 nirS 杆菌 Acidovorax 和 Azospirillum 的相对丰度，而增加了 nirK 杆菌 Rhodopseudomonas 和 Bosea。曼特尔检验表明，甘肃虫感染降低了硝化细菌群落与土壤特性之间的相关性。结论该研究证明了甘肃鹅膏菌在不同P营养条件下宿主草分解过程中对硝化细菌和反硝化细菌群落的重要作用，有助于我们全面了解鹅膏菌内生菌的生态意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Plant and Soil 农林科学-农艺学

CiteScore

8.20

自引率

8.20%

发文量

543

审稿时长

2.5 months

期刊介绍： Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.