Deciphering the biodegradation of thiamethoxam by Phanerochaete chrysosporium with natural siderite: synergistic mechanisms, transcriptomics characterization, and molecular simulation

IF 12.2 1区 环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL
Shiye Zhu, Anwei Chen, Jiale Zhang, Si Luo, Jizhao Yang, Youzheng Chai, Jianhua Zeng, Ma Bai, Zhenghang Yang, Gen Lu
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Abstract

Fungi play vital roles in the fate of organic pollutants, particularly when interacting with minerals in aquatic and soil environments. Mechanisms by which fungi may mitigate pollutions in fungus-mineral interactions are still unclear. Inspired by biogeochemical cycling, we constructed a range of co-culture systems to investigate synergistic effects of the white-rot fungus Phanerochaete chrysosporium and the iron-bearing mineral siderite on thiamethoxam (THX) transformation, a common neonicotinoid pesticide. Co-culturing with siderite significantly enhanced THX transformation during the initial 10 days with a dose effect, achieving 86% removal within 25 days. Fungi could affect siderite’s dissolution, transformation, and precipitation through their biological activities. These interactions triggered physiological adaptation and resilience in fungi. Siderite could enhance the activity of fungal ligninolytic enzymes and cytochrome P450, facilitating biotransformation. Genes expression related to growth, energy metabolism, and oxidative stress response upregulated, enhancing fungal resilience to THX. The primary THX degradation pathways included nitro-reduction, C-N cleavage, and de-chlorination. Molecular dynamics simulations provided insights into catalytic mechanisms of enzyme-THX interactions. Together, siderite could act as natural enhancers that endowed fungi to resist physical and chemical stresses in environments, providing insights into contaminants attenuation, fungal biomineralization, and the coevolution of the Earth's lithosphere and biosphere.

Abstract Image

解密蛹虫草菌与天然菱铁矿对噻虫嗪的生物降解:协同机制、转录组学表征和分子模拟
真菌在有机污染物的归宿中发挥着至关重要的作用,尤其是在水生和土壤环境中与矿物质相互作用时。在真菌与矿物质的相互作用中,真菌减轻污染的机制尚不清楚。受生物地球化学循环的启发,我们构建了一系列共培养系统,研究白腐真菌 Phanerochaete chrysosporium 和含铁矿物菱铁矿对噻虫嗪(THX)(一种常见的新烟碱类农药)转化的协同效应。在最初的 10 天内,与菱铁矿共同培养能显著提高 THX 的转化率,并产生剂量效应,在 25 天内达到 86% 的去除率。真菌可通过其生物活性影响菱铁矿的溶解、转化和沉淀。这些相互作用引发了真菌的生理适应和恢复能力。菱铁矿可以增强真菌木质素分解酶和细胞色素 P450 的活性,促进生物转化。与生长、能量代谢和氧化应激反应有关的基因表达上调,增强了真菌对 THX 的适应能力。THX 的主要降解途径包括硝基还原、C-N 裂解和脱氯。分子动力学模拟深入揭示了酶与 THX 相互作用的催化机理。总之,菱铁矿可以作为天然的增强剂,赋予真菌抵抗环境中物理和化学压力的能力,为污染物衰减、真菌生物矿化以及地球岩石圈和生物圈的共同演化提供启示。
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来源期刊
Journal of Hazardous Materials
Journal of Hazardous Materials 工程技术-工程:环境
CiteScore
25.40
自引率
5.90%
发文量
3059
审稿时长
58 days
期刊介绍: The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.
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