Effective Treatment and Biodegradation Mechanism Analysis of Petroleum Hydrocarbon Wastewater by Immobilized Ochrobactrum sp. WY-4 on Iron-modified Biochar

IF 3 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Water, Air, & Soil Pollution Pub Date : 2024-10-25 DOI:10.1007/s11270-024-07574-8

Hengyu Chen, Chuan Yin, Fei Su, Jiancong He, Songling Wu, Menghan Jiang, Huanfang Gao, Cong Li

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Abstract

Total petroleum hydrocarbon (TPH) in wastewater has attracted widespread attention for its environmental and biological health hazards. In the research, WY-4 strains with diesel degradation ability isolated from contaminated soil and response surface methodology was used to optimize the degradation conditions of WY-4. Fe-modified biochar (FPB) was used as an immobilized carrier, the environmental factors affecting the degradation of immobilized bacteria (FPBM) were explored and the degradation effect of FPBM was evaluated on real TPH-contaminated wastewater. Furthermore, the potential degradation mechanisms and possible degradation pathways of TPH were also explored. The results demonstrated that WY-4 was identified as Ochrobactrum sp., and its optimal growth conditions were pH 6.8, temperature 28.8°C and NaCl concentration 9.47 g/L. The removal efficiency by FPBM on 10,000 mg/L diesel wastewater was 72.5% and on real TPH-contaminated wastewater was 76.75% in 7 d, which was significantly higher than the degradation effect of free bacteria. The degradation pathway of two representative pollutants, naphthalene and indole, in the real TPH-contaminated wastewater was referred to be the catechol metabolic pathway. The results highlighted the potential of FPB-immobilized bacteria for the remediation of TPH-contaminated wastewater in harsh environments and provided an effective strategy for green remediation treatment of TPH contamination.

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铁改性生物炭上固定化赭菌 WY-4 对石油烃废水的有效处理及生物降解机理分析

废水中的总石油烃（TPH）因其对环境和生物健康的危害而受到广泛关注。本研究从污染土壤中分离出具有柴油降解能力的 WY-4 菌株，并采用响应面方法优化了 WY-4 的降解条件。采用铁改性生物炭（FPB）作为固定化载体，探讨了影响固定化细菌（FPBM）降解的环境因素，并评估了 FPBM 对实际 TPH 污染废水的降解效果。此外，还探讨了 TPH 的潜在降解机制和可能的降解途径。结果表明，WY-4 被鉴定为 Ochrobactrum sp.，其最佳生长条件为 pH 6.8、温度 28.8°C、NaCl 浓度 9.47 g/L。在 7 d 内，FPBM 对 10,000 mg/L 柴油废水的去除率为 72.5%，对实际 TPH 污染废水的去除率为 76.75%，明显高于游离菌的降解效果。实际 TPH 污染废水中萘和吲哚这两种代表性污染物的降解途径被认为是儿茶酚代谢途径。研究结果凸显了 FPB 固定化细菌在恶劣环境下修复 TPH 污染废水的潜力，为 TPH 污染的绿色修复处理提供了有效策略。

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来源期刊

Water, Air, & Soil Pollution 环境科学-环境科学

CiteScore

4.50

自引率

6.90%

发文量

448

审稿时长

2.6 months

期刊介绍： Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments. Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation. Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.