Electrochemical impedimetric aptasensor for the detection of Urea based on oxidized graphitic carbon nitride utilizing flexible electrode platform

IF 2.8 4区工程技术 Q2 ENGINEERING, ELECTRICAL & ELECTRONIC

Journal of Materials Science: Materials in Electronics Pub Date : 2024-10-28 DOI:10.1007/s10854-024-13711-0

Annu Mishra, Pradakshina Sharma, Mohd. Rahil Hasan, Arun Kumar, Souradeep Roy, Manika Khanuja, Jagriti Narang

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引用次数: 0

Abstract

Urea is a significant potential biomarker of kidney and liver-related diseases. Numerous traditional approaches are available to detect Urea. All of them are sensitive and specific but have substantial setbacks, such as being expensive, requiring expertise and taking time to bring results. Therefore in the present study, an electrochemical biosensor was designed using printed carbon conductive ink (PCCI)-based flexible electrode and integrated with aptamer and oxidized graphitic carbon nitride (Ox-g-C3N4) to detect Urea in samples. Electrochemical validation of the designed aptasensor was done by CV, DPV, and EIS. The designed aptasensor exhibited wide linear variety (20–100 mM) with 20 mM LOD and showed anti-interference ability as results proved that it was highly selective towards Urea. Using flexible electrodes made the sensor more economical and required significantly less reagent/analysis volume. Furthermore, aptamers are more stable than enzymes, making the sensor more robust and specific towards urea detection.

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利用柔性电极平台、基于氧化石墨氮化碳的电化学阻抗传感器检测尿素

尿素是肾脏和肝脏相关疾病的重要潜在生物标志物。目前有许多传统方法可用于检测尿素。所有这些方法都具有灵敏性和特异性，但也有很大的缺陷，如价格昂贵、需要专业技术和需要很长时间才能得出结果。因此，本研究设计了一种电化学生物传感器，使用基于印刷碳导电墨水（PCCI）的柔性电极，并集成了适配体和氧化石墨氮化碳（Ox-g-C3N4），用于检测样品中的尿素。通过 CV、DPV 和 EIS 对设计的适配体进行了电化学验证。结果表明，所设计的适配传感器对尿素具有高度选择性，表现出较宽的线性范围（20-100 毫摩尔）和 20 毫摩尔的 LOD，并显示出抗干扰能力。使用柔性电极使传感器更加经济，所需的试剂/分析体积大大减少。此外，适配体比酶更稳定，使传感器对尿素的检测更稳定、更特异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Materials Science: Materials in Electronics 工程技术-材料科学：综合

CiteScore

5.00

自引率

7.10%

发文量

1931

审稿时长

2 months

期刊介绍： The Journal of Materials Science: Materials in Electronics is an established refereed companion to the Journal of Materials Science. It publishes papers on materials and their applications in modern electronics, covering the ground between fundamental science, such as semiconductor physics, and work concerned specifically with applications. It explores the growth and preparation of new materials, as well as their processing, fabrication, bonding and encapsulation, together with the reliability, failure analysis, quality assurance and characterization related to the whole range of applications in electronics. The Journal presents papers in newly developing fields such as low dimensional structures and devices, optoelectronics including III-V compounds, glasses and linear/non-linear crystal materials and lasers, high Tc superconductors, conducting polymers, thick film materials and new contact technologies, as well as the established electronics device and circuit materials.