Implementation of an Upflow Fixed Bed Bioreactor for Denitrification Coupled to Methane Oxidation: Performance and Biomass Development Under Anoxic Conditions
Anngie K. Molina-Macías, Yudy Andrea Londoño, Nancy Pino, Gustavo A. Peñuela
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引用次数: 0
Abstract
Denitrification coupled to methane oxidation (DOM) has been shown to be an appropriate process for wastewater treatment applications, since it can reduce greenhouse gas emissions and nitrogen discharges, making wastewater treatment plants more environmentally sustainable. Study of DOM has focused on laboratory-scale application using membrane biological reactors (MBR) or sequency batch reactors (SBR), which have been shown to be able to retain DOM biomass and therefore appropriate for use with this process. However, it is necessary to expand knowledge of the behavior of this process using other configurations, with a view to scaling up. Therefore, in this study, an upflow fixed bed bioreactor (UFBR) was implemented using plastic carriers such as bioballs and Biochips® to carry out the DOM process under anoxic conditions. The reactor reached stable nitrogen removal conditions after approximately 400 days of continuous operation, forming a biomass composed of denitrifying methane-oxidizing microorganisms where the genus Anaerolinea and Methylocystis predominated. Once the biomass was formed and the DOM process was stabilized, maximum nitrite and nitrate removal rates of 17.6 mgN-NO2−/L-d and 8.9 mgN-NO3−/L-d, respectively, and a removal efficiency of methane up to 77% were obtained. This demonstrates the feasibility of the application of the DOM process under anoxic conditions using fixed bed bioreactors, which is promising for further nitrogen removal from wastewater using a varied reactor configuration easily to scaled-up.
反硝化耦合甲烷氧化(DOM)已被证明是一种适合废水处理应用的工艺,因为它可以减少温室气体排放和氮排放,使废水处理厂更具环境可持续性。对 DOM 的研究主要集中在使用膜生物反应器(MBR)或序批式反应器(SBR)进行实验室规模的应用,这些反应器已被证明能够保留 DOM 生物质,因此适合用于该工艺。不过,有必要扩大对使用其他配置的该工艺行为的了解,以便扩大规模。因此,在本研究中,采用了一种上流式固定床生物反应器(UFBR),使用生物球和 Biochips® 等塑料载体,在缺氧条件下进行 DOM 处理。反应器在连续运行约 400 天后达到了稳定的脱氮条件,形成了由反硝化甲烷氧化微生物组成的生物量,其中以 Anaerolinea 和 Methylocystis 属微生物为主。生物量形成和 DOM 过程稳定后,亚硝酸盐和硝酸盐的最大去除率分别达到 17.6 mgN-NO2-/L-d 和 8.9 mgN-NO3-/L-d,甲烷去除率高达 77%。这表明在缺氧条件下使用固定床生物反应器应用 DOM 工艺是可行的,而且这种反应器结构多样,易于放大,有望进一步去除废水中的氮。
期刊介绍:
Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments.
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Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.