Enhancing osteogenesis and mandibular defect repair with magnesium-modified acellular bovine bone matrix

IF 4.2 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Peng Wang, Rui Ge, Biao Li, Li Li, Liwei Han, Xiantong Hu, Gang Xu, Kaitao Yu
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引用次数: 0

Abstract

An acellular bovine bone matrix modified to release Magnesium ions (Mg2+) (ABBM-Mg) was prepared and evaluated for its potential in osteogenesis and mandibular defect repair. Mg2+ was incorporated into ABBM using an ion exchange method. The microstructure and mechanical properties of both ABBM and ABBM-Mg were analyzed using SEM and a biomechanical testing machine. Cytocompatibility, cell adhesion, and osteogenic differentiation were assessed using various methods including CCK-8, Live/Dead staining, SEM, ALP staining, and qPCR analysis in MC3T3-E1 cells. Additionally, a mandibular defect model in rats was established. The bone defect repair outcomes were evaluated using Micro-CT, histological HE staining, and Masson staining. The study showed that mineralization containing magnesium was redeposited on the surface of the three-dimensional porous ABBM, and the ABBM-Mg scaffold promoted cell proliferation and osteogenic differentiation compared to the ABBM scaffold. In the rat mandibular defect model, the ABBM-Mg scaffold demonstrated superior bone repair ability. This study successfully incorporated Mg2+ into ABBM without significantly affecting its microstructure and compressive strength. Furthermore, ABBM-Mg showed sustained release of Mg2+ which enhanced cell proliferation, adhesion, and osteogenic differentiation in vitro, and promoted mandibular defect healing in rats. This research opens up new possibilities for the clinical application of functionalized acellular bone matrix.

Graphical Abstract

用镁改性细胞状牛骨基质促进成骨和下颌骨缺损修复
我们制备了一种可释放镁离子(Mg2+)的无细胞牛骨基质(ABBM-Mg),并对其在成骨和下颌骨缺损修复方面的潜力进行了评估。采用离子交换法将 Mg2+ 加入 ABBM。使用扫描电镜和生物力学试验机分析了 ABBM 和 ABBM-Mg 的微观结构和力学性能。在 MC3T3-E1 细胞中使用多种方法评估了细胞相容性、细胞粘附性和成骨分化,包括 CCK-8、活/死染色、SEM、ALP 染色和 qPCR 分析。此外,还建立了大鼠下颌骨缺损模型。使用 Micro-CT、组织学 HE 染色和 Masson 染色对骨缺损修复结果进行了评估。研究表明,三维多孔 ABBM 表面重新沉积了含镁的矿化物,与 ABBM 支架相比,ABBM-镁支架促进了细胞增殖和成骨分化。在大鼠下颌骨缺损模型中,ABBM-Mg 支架表现出更优越的骨修复能力。这项研究成功地将 Mg2+ 添加到 ABBM 中,而不会明显影响其微观结构和抗压强度。此外,ABBM-Mg 还能持续释放 Mg2+,从而增强体外细胞增殖、粘附和成骨分化,促进大鼠下颌骨缺损愈合。这项研究为功能化无细胞骨基质的临床应用提供了新的可能性。
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来源期刊
Journal of Materials Science: Materials in Medicine
Journal of Materials Science: Materials in Medicine 工程技术-材料科学:生物材料
CiteScore
8.00
自引率
0.00%
发文量
73
审稿时长
3.5 months
期刊介绍: The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.
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