The prevalence, antimicrobial susceptibility, virulence, and antimicrobial resistance genes of multidrug-resistant Vibrio parahaemolyticus recovered from Oreochromis niloticus

IF 2.2 3区农林科学 Q2 FISHERIES

Aquaculture International Pub Date : 2024-08-01 DOI:10.1007/s10499-024-01625-x

Abdelazeem M. Algammal, Mahmoud Mabrok, Khyreyah J. Alfifi, Saad Alghamdi, Enas A. Almanzalawi, Tahani M. Alqahtani, Mahmoud E. Elsayed, Hanan Elghayaty, Zainab Mohamed El Kattawy, Reham M. El-Tarabili

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Abstract

Vibrio parahaemolyticus is associated with severe food-borne illness in humans as well as substantial financial setbacks in the fishing sector. To investigate the prevalence, pathogenicity, virulence, as well as the resistance patterns of V. parahaemolyticus retrieved from Oreochromis niloticus, 180 O. niloticus (moribund fish; n = 120 and apparently healthy; n = 60) were indiscriminately collected from Lake Manzala, Egypt. Consequently, the clinical inspection, necropsy finding, and bacteriological analysis were performed. All the retrieved isolates tested positive for the groEL species-specific gene. Herein, the prevalence of V. parahaemolyticus in the collected fish was 8.9% (16/180), whereas the liver was the organ most prominently affected. PCR revealed that the most prevalent virulence-determinant genes associated with V. parahaemolyticus were the toxR and tlh genes (100% for each), then tdh (73.3%) and trh (26.7%) genes. Furthermore, 40% (18/45) of isolated V. parahaemolyticus were extensively drug-resistant (XDR) to seven antimicrobial classes and have bla_TEM, bla_OXA, sul1, aadA, ermB, and tetA genes. Additionally, 17.8% (8/45) of the obtained V. parahaemolyticus were multidrug-resistant (MDR) to three antimicrobial classes and owned bla_TEM, ermB, and aadA genes. The pathogenicity assay accentuated the correlation between cumulative mortality and the virulent characteristics exhibited by the inoculated strain. Definitely, 77% mortality was recorded within eight days in fish injected with a virulent V. parahaemolyticus strain. Briefly, this research accentuated the occurrence of XDR V. parahaemolyticus in fish specifying a public health concern. The emerging MDR and XDR V. parahaemolyticus frequently harbored toxR, tlh, and tdh virulence genes, and bla_TEM, bla_OXA, sul1, aadA, ermB, and tetA or tetB antimicrobial resistance genes.

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从黑鲷鱼中发现的耐多药副溶血性弧菌的流行率、抗菌药敏感性、毒力和抗菌药耐药基因

副溶血性弧菌与人类的严重食源性疾病以及渔业的巨大经济损失有关。为了研究从黑线鲈体内检出的副溶血性弧菌的流行率、致病性、毒力和抗药性模式，研究人员从埃及曼扎拉湖不加区分地采集了 180 尾黑线鲈（病死鱼 120 尾，明显健康鱼 60 尾）。因此，对这些鱼进行了临床检查、尸体解剖和细菌学分析。所有检测到的分离物均对 groEL 的物种特异性基因检测呈阳性。其中，副溶血性弧菌在所采集鱼类中的感染率为 8.9%（16/180），而肝脏是受影响最严重的器官。聚合酶链式反应（PCR）显示，副溶血性弧菌最常见的毒力决定基因是toxR和tlh基因（各占100%），然后是tdh（73.3%）和trh（26.7%）基因。此外，40%（18/45）分离出的副溶血性弧菌对七种抗菌药物广泛耐药（XDR），并带有 blaTEM、blaOXA、sul1、aadA、ermB 和 tetA 基因。此外，17.8%（8/45）的副溶血性弧菌对三种抗菌药物具有多重耐药性（MDR），并拥有 blaTEM、ermB 和 aadA 基因。致病性试验表明，累积死亡率与接种菌株表现出的毒性特征之间存在关联。具体而言，注射了毒力强的副溶血性弧菌菌株的鱼类在八天内的死亡率达到 77%。简而言之，这项研究强调了副溶血性弧菌 XDR 在鱼类中的出现，引起了公共卫生关注。新出现的 MDR 和 XDR 副溶血性弧菌经常携带 toxR、thalh 和 tdh 毒力基因，以及 blaTEM、blaOXA、sul1、aadA、ermB 和 tetA 或 tetB 抗菌药耐药性基因。

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来源期刊

Aquaculture International 农林科学-渔业

CiteScore

5.10

自引率

6.90%

发文量

204

审稿时长

1.0 months

期刊介绍： Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.