A rapid field-ready electrical biosensor consisting of bismuthine-derived Au island decorated BiOCl nanosheets for Raphidiopsis raciborskii detection in freshwater†

IF 6.1 3区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS
Hyunjun Park, Sun Woo Kim, Siyun Lee, Jeongyun An, Seokho Jung, Minju Lee, Jeonghyun Kim, Daeryul Kwon, Hongje Jang and Taek Lee
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Abstract

Cyanobacteria play an essential role in nutrient cycling in aquatic ecosystems. However, certain species adversely affect the environment and human health by causing harmful cyanobacterial algal blooms (cyanoHABs) and producing cyanotoxins. To address this issue, continuous cyanoHAB monitoring has been considered; however, a gold standard has not yet been established. In this study, we aimed to develop a dual DNA-targeting capacitive-type biosensor for rapid field-ready monitoring of Raphidiopsis raciborskii, a causative species of cyanoHAB. To enhance the sensing signal, a plate-like AuBiOCl nanocomposite was synthesized using a spontaneous carbonation process without additional additives. The alternating-current electrothermal flow (ACEF) technique was applied to enable rapid DNA and probe binding within 10 min. The limits of detection (LODs) for R. raciborskii RubisCO large subunit (rbcL) and RNA polymerase beta subunit (rpoB) genes diluted in deionized (DI) water were 4.89 × 10−17 and 3.89 × 10−17 M, respectively. Furthermore, the LODs of R. raciborskii rbcl and rpoB diluted in freshwater containing HAB were 2.55 × 10−16 and 3.84 × 10−16 M, respectively, demonstrating the field-ready applicability of the device. The fabricated cyanobacterial DNA-sensing platform enabled powerful species-specific detection using a small sample volume and low target concentration without a nucleic acid amplification step, dramatically reducing the detection time. This study has considerable implications for detecting HABs, early warning systems, and species-specific environmental monitoring technology.

Abstract Image

一种可在现场快速使用的电生物传感器,由铋源金岛装饰的 BiOCl 纳米片组成,用于检测淡水中的 Raphidiopsis raciborskii。
蓝藻在水生生态系统的营养循环中发挥着重要作用。然而,某些种类的蓝藻会引起有害的蓝藻藻华(cyanoHABs)并产生蓝藻毒素,从而对环境和人类健康造成不利影响。为解决这一问题,人们考虑对蓝藻藻华进行连续监测;然而,目前尚未建立金标准。在本研究中,我们旨在开发一种双 DNA 靶向电容式生物传感器,用于现场快速监测 Raphidiopsis raciborskii(一种 cyanoHAB 的致病物种)。为了增强传感信号,研究人员采用自发碳化工艺合成了一种板状金-BiOCl 纳米复合材料,没有添加其他添加剂。交变电流电热流(ACEF)技术可在 10 分钟内实现 DNA 与探针的快速结合。在去离子水中稀释的 R. raciborskii RubisCO 大亚基(rbcL)和 RNA 聚合酶 beta 亚基(rpoB)基因的检测限(LOD)分别为 4.89 × 10-17 M 和 3.89 × 10-17 M。此外,在含有 HAB 的淡水中稀释的 R. raciborskii rbcl 和 rpoB 的 LOD 分别为 2.55 × 10-16 M 和 3.84 × 10-16 M,这表明该装置可用于现场。该蓝藻 DNA 传感平台无需核酸扩增步骤,只需较小的样品量和较低的目标浓度即可实现强大的物种特异性检测,大大缩短了检测时间。这项研究对检测有害藻华、预警系统和物种特异性环境监测技术具有重要意义。
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来源期刊
Journal of Materials Chemistry B
Journal of Materials Chemistry B MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
11.50
自引率
4.30%
发文量
866
期刊介绍: Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive: Antifouling coatings Biocompatible materials Bioelectronics Bioimaging Biomimetics Biomineralisation Bionics Biosensors Diagnostics Drug delivery Gene delivery Immunobiology Nanomedicine Regenerative medicine & Tissue engineering Scaffolds Soft robotics Stem cells Therapeutic devices
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