Polyethylene microplastic modulates the toxicity of pentachlorophenol to the microalgae Isochrysis galbana, clone t-ISO

Abstract

Pentachlorophenol (PCP) and polyethylene microplastic (PE-MP) have been designated as emerging and persistent pollutants, respectively. The combined effects of those pollutants are still unknown, especially to organisms like phytoplankton that may adsorb to their surface. Therefore, the purpose of this study was to investigate for the first time the effects of PE-MP alone and in combination with PCP on the microalgae Isochrysis galbana, clone t-ISO following 72 h of exposure. Photosynthetic pigments amounts, carotenoid, protein, carbohydrate and fatty acids have been assessed. Acute toxicity test showed that the 72 h median inhibition concentration (72 h-EC₅₀) was 148.2, 0.66 and 087 mg L⁻¹ for PE-MP, PCP and their mixture. The utmost effects in growth inhibition rates were noted with 0.5 and 1.25 mg L⁻¹ PCP (23% and 85%, respectively), and 100 and 300 mg L⁻¹ PE-MP (49% and 64%, respectively). Moreover, it was found that those concentrations had a major impact on the photosynthetic pigments, protein, carbohydrate, and fatty acids amounts in algal cells. Furthermore, levels of H₂O₂ and Malondialdehyde (MDA), as well as the activities of catalase (CAT), superoxide dismutase (SOD), and ascorbate peroxidase (APX), indicated the induction of an oxidative stress in algal cells. It appears that adding PE-MP at a no-effect concentration (25 mg L⁻¹) reduces the toxicity caused by PCP due to its adsorption to polyethylene microplastics.