Development and implementation of a simulated microgravity setup for edible cyanobacteria.

IF 4.4 1区物理与天体物理 Q1 MULTIDISCIPLINARY SCIENCES

npj Microgravity Pub Date : 2024-10-25 DOI:10.1038/s41526-024-00436-x

Gabriele Ellena, Jana Fahrion, Surya Gupta, Claude-Gilles Dussap, Arianna Mazzoli, Natalie Leys, Felice Mastroleo

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Abstract

Regenerative life support systems for space crews recycle waste into water, food, and oxygen using different organisms. The European Space Agency's MELiSSA program uses the cyanobacterium Limnospira indica PCC8005 for air revitalization and food production. Before space use, components' compatibility with reduced gravity was tested. This study introduced a ground analog for microgravity experiments with oxygenic cyanobacteria under continuous illumination, using a random positioning machine (RPM) setup. L. indica PCC8005 grew slower under low-shear simulated microgravity, with proteome analysis revealing downregulation of ribosomal proteins, glutamine synthase, and nitrate uptake transporters, and upregulation of gas vesicle, photosystem I and II, and carboxysome proteins. Results suggested inhibition due to high oxygen partial pressure, causing carbon limitation when cultivated in low-shear simulated microgravity. A thicker stagnant fluid boundary layer reducing oxygen release in simulated microgravity was observed. These findings validate this RPM setup for testing the effects of non-terrestrial gravity on photosynthetic microorganisms.

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开发和实施可食用蓝藻的模拟微重力装置。

太空乘员的再生生命支持系统利用不同的生物将废物回收为水、食物和氧气。欧洲航天局的 MELiSSA 计划利用蓝藻菌 Limnospira indica PCC8005 进行空气活化和食物生产。在太空使用之前，要对组件与重力降低的兼容性进行测试。本研究采用随机定位机（RPM）装置，对连续光照下的含氧蓝藻进行了地面模拟微重力实验。在低剪切力模拟微重力条件下，L. indica PCC8005 的生长速度较慢，蛋白质组分析显示核糖体蛋白、谷氨酰胺合成酶和硝酸盐吸收转运体下调，而气体囊、光系统 I 和 II 以及羧酶体蛋白上调。结果表明，在低剪切模拟微重力条件下培养时，高氧分压导致碳限制，从而造成抑制。在模拟微重力条件下，观察到较厚的停滞流体边界层减少了氧气的释放。这些发现验证了这种 RPM 设置可用于测试非地球重力对光合微生物的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

npj Microgravity Physics and Astronomy-Physics and Astronomy (miscellaneous)

CiteScore

7.30

自引率

7.80%

发文量

审稿时长

9 weeks

期刊介绍： A new open access, online-only, multidisciplinary research journal, npj Microgravity is dedicated to publishing the most important scientific advances in the life sciences, physical sciences, and engineering fields that are facilitated by spaceflight and analogue platforms.