Integration of fungal transcriptomics and metabolomics provides insights into the early interaction between the ORM fungus Tulasnella sp. and the orchid Serapias vomeracea seeds.

IF 5.2 1区 生物学 Q1 MYCOLOGY
Silvia De Rose, Fabiano Sillo, Andrea Ghirardo, Silvia Perotto, Jörg-Peter Schnitzler, Raffaella Balestrini
{"title":"Integration of fungal transcriptomics and metabolomics provides insights into the early interaction between the ORM fungus Tulasnella sp. and the orchid Serapias vomeracea seeds.","authors":"Silvia De Rose, Fabiano Sillo, Andrea Ghirardo, Silvia Perotto, Jörg-Peter Schnitzler, Raffaella Balestrini","doi":"10.1186/s43008-024-00165-6","DOIUrl":null,"url":null,"abstract":"<p><p>In nature, germination of orchid seeds and early plant development rely on a symbiotic association with orchid mycorrhizal (ORM) fungi. These fungi provide the host with the necessary nutrients and facilitate the transition from embryos to protocorms. Despite recent advances in omics technologies, our understanding of this symbiosis remains limited, particularly during the initial stages of the interaction. To address this gap, we employed transcriptomics and metabolomics to investigate the early responses occurring in the mycorrhizal fungus Tulasnella sp. isolate SV6 when co-cultivated with orchid seeds of Serapias vomeracea. The integration of data from gene expression and metabolite profiling revealed the activation of some fungal signalling pathways before the establishment of the symbiosis. Prior to seed contact, an indole-related metabolite was produced by the fungus, and significant changes in the fungal lipid profile occurred throughout the symbiotic process. Additionally, the expression of plant cell wall-degrading enzymes (PCWDEs) was observed during the pre-symbiotic stage, as the fungus approached the seeds, along with changes in amino acid metabolism. Thus, the dual-omics approach employed in this study yielded novel insights into the symbiotic relationship between orchids and ORM fungi and suggest that the ORM fungus responds to the presence of the orchid seeds prior to contact.</p>","PeriodicalId":54345,"journal":{"name":"Ima Fungus","volume":"15 1","pages":"31"},"PeriodicalIF":5.2000,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11503967/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ima Fungus","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1186/s43008-024-00165-6","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

In nature, germination of orchid seeds and early plant development rely on a symbiotic association with orchid mycorrhizal (ORM) fungi. These fungi provide the host with the necessary nutrients and facilitate the transition from embryos to protocorms. Despite recent advances in omics technologies, our understanding of this symbiosis remains limited, particularly during the initial stages of the interaction. To address this gap, we employed transcriptomics and metabolomics to investigate the early responses occurring in the mycorrhizal fungus Tulasnella sp. isolate SV6 when co-cultivated with orchid seeds of Serapias vomeracea. The integration of data from gene expression and metabolite profiling revealed the activation of some fungal signalling pathways before the establishment of the symbiosis. Prior to seed contact, an indole-related metabolite was produced by the fungus, and significant changes in the fungal lipid profile occurred throughout the symbiotic process. Additionally, the expression of plant cell wall-degrading enzymes (PCWDEs) was observed during the pre-symbiotic stage, as the fungus approached the seeds, along with changes in amino acid metabolism. Thus, the dual-omics approach employed in this study yielded novel insights into the symbiotic relationship between orchids and ORM fungi and suggest that the ORM fungus responds to the presence of the orchid seeds prior to contact.

真菌转录组学和代谢组学的整合有助于深入了解ORM真菌Tulasnella sp.与兰花Serapias vomeracea种子之间的早期相互作用。
在自然界中,兰花种子的萌发和植物的早期发育依赖于与兰花菌根真菌(ORM)的共生关系。这些真菌为宿主提供必要的养分,并促进胚胎向原生质体的过渡。尽管最近在全息技术方面取得了进展,但我们对这种共生关系的了解仍然有限,尤其是在相互作用的初始阶段。为了填补这一空白,我们利用转录组学和代谢组学研究了菌根真菌 Tulasnella sp.通过整合基因表达和代谢物分析数据,发现在共生关系建立之前,一些真菌信号通路已经被激活。在与种子接触之前,真菌产生了一种吲哚相关代谢物,在整个共生过程中,真菌脂质谱发生了显著变化。此外,在共生前阶段,当真菌接近种子时,还观察到植物细胞壁降解酶(PCWDEs)的表达以及氨基酸代谢的变化。因此,本研究采用的双重组学方法对兰花与 ORM 真菌之间的共生关系有了新的认识,并表明 ORM 真菌在接触兰花种子之前会对种子的存在做出反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Ima Fungus
Ima Fungus Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
11.00
自引率
3.70%
发文量
18
审稿时长
20 weeks
期刊介绍: The flagship journal of the International Mycological Association. IMA Fungus is an international, peer-reviewed, open-access, full colour, fast-track journal. Papers on any aspect of mycology are considered, and published on-line with final pagination after proofs have been corrected; they are then effectively published under the International Code of Nomenclature for algae, fungi, and plants. The journal strongly supports good practice policies, and requires voucher specimens or cultures to be deposited in a public collection with an online database, DNA sequences in GenBank, alignments in TreeBASE, and validating information on new scientific names, including typifications, to be lodged in MycoBank. News, meeting reports, personalia, research news, correspondence, book news, and information on forthcoming international meetings are included in each issue
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信