Molecular detection of Coxiella burnetii infection (Q fever) in livestock in Makkah Province, Saudi Arabia.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-10-24 DOI:10.1515/znc-2024-0126

Hassan M Baroom, Naser A Alkenani, Bassam O Al-Johny, Adi A Almohimeed, Mohammed S Mohammed, Layla A Alshehri, Shaker S Althobaiti, Raga I Omar, Majed A Alshaeri, Saleh M Al-Mmaqar

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Abstract

The study aims to investigate the prevalence of Q fever in livestock and ticks in Makkah Province, Saudi Arabia, by molecular methods. Using DNA obtained from (40) blood samples, (60) vaginal swabs and ticks (120) samples. Real-time PCR was used to detect the IS1111 insertion sequence of Coxiella burnetii in aborted animals. Among 40 blood samples only one sample of the camel was found to be infected with an overall prevalence of 2.5 %. The highest prevalence (10 %) was recorded in AL-Laith in one camel blood sample out of 10 samples examined. Of 60 vaginal swabs examined for C. burnetii DNA, four samples were found to be infected with an overall prevalence of 6.6 %. The highest prevalence (10 %) was recorded in Makkah in two camel vaginal swabs out of 20 samples, followed by Jeddah and AL-Laith with a prevalence of (5.6 %) by detection of one sample positive out of 18 samples on each of them, while vaginal swabs from AL-Kamil were negative. Three types of ticks were identified Hyalomma dromedarii, Hyalomma anatolicum, and Hyalomma excavatum. H. dromedarii tick is the most common in aborted camels with a prevalence (6.7 %) in Makkah followed by Jeddah (5 %). The findings of this study revealed that C. burnetii infection is prevalent in agricultural animals especially camels and ticks maintained at livestock farms in Makkah Province. However, these animals and ticks may pass on C. burnetii infections to nearby people and other animals in the study area.

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沙特阿拉伯麦加省牲畜烧伤柯西氏菌感染（Q 热）的分子检测。

本研究旨在通过分子方法调查 Q 热在沙特阿拉伯麦加省牲畜和蜱虫中的流行情况。使用从（40 份）血液样本、（60 份）阴道拭子样本和（120 份）蜱样本中获得的 DNA。使用实时 PCR 检测流产动物体内烧伤柯西氏菌的 IS1111 插入序列。在 40 份血液样本中，只发现一份骆驼样本受到感染，总感染率为 2.5%。在 AL-Laith 检测的 10 份骆驼血液样本中，一份样本的感染率最高（10%）。在对 60 份阴道拭子进行的烧伤梭菌 DNA 检测中，发现 4 份样本受到感染，总感染率为 6.6%。麦加的感染率最高（10%），在 20 个骆驼阴道拭子样本中发现了两个样本；其次是吉达和 AL-Laith，在 18 个样本中各发现了一个阳性样本，感染率为 5.6%；而 AL-Kamil 的阴道拭子样本呈阴性。确定了三种蜱虫：Hyalomma dromedarii、Hyalomma anatolicum 和 Hyalomma excavatum。H. dromedarii 是流产骆驼中最常见的蜱虫，在麦加的发病率为 6.7%，其次是吉达（5%）。这项研究结果表明，烧伤蜱感染在麦加省的农业动物中很普遍，尤其是骆驼和畜牧场饲养的蜱虫。不过，这些动物和蜱虫可能会将烧伤蜱传给附近的人和研究地区的其他动物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.