Effect of Lactobacillus acidophilus, Calcium, and Moringa oleifera leaves extract co-administration can prevent chemical-induced carcinogenesis.

Abstract

Background: Colon cancer is the fourth leading cause of cancer deaths worldwide. The present study evaluated the chemopreventive effect of the combined treatment of Lactobacillus acidophilus, calcium citrate, and Moringa oleifera leaves extract against DMH (1,1-dimethylhydrazine hydrochloride) induced colon cancer.

Material and methods: Sprague Dawley rats were grouped into 10 different groups and treated with DMH 30 mg/kg s.c. for 8 weeks, Successful induction of colon cancer was confirmed with the help of symptoms, Individual and combined treatments of Lactobacillus acidophilus (10⁹ cfu p.o.), calcium citrate (2 mg/kg p.o.) and Moringa oleifera (100 & 200 mg/kg p.o.) leaves extracts were used for 4 weeks.

Result: After 4 weeks of treatment, it was observed that a significant reduction in aberrant crypt foci (ACFs) count, whereas liver and kidney function, AST (aspartate transaminase), ALT (alanine transaminase), urea and creatinine biomarkers were retained in its normal range. Upon DMH treatment, liver tissue loses histoarchitecture with mononuclear cell infiltration, nuclear enlargement, and hyperchromasia, this reverts due to the combined treatment of Lactobacillus acidophilus (LA), calcium citrate (CC) and hydroalcoholic extract of Moringa oleifera leaves (ME). From the results, it was revealed that individual and combined treatment of Lactobacillus acidophilus, calcium citrate, and hydroalcoholic extract of Moringa oleifera leaves shows beneficial effects against the carcinogen.

Conclusion: Combined treatment of Lactobacillus acidophilus, calcium citrate, and hydroalcoholic extract of Moringa oleifera leaves showed positive effects against carcinogenesis and lowered aberrant crypt foci count and shows histoarchitectural improvements in liver histology with no nuclear enlargement and hyperchromasia in liver tissue.