Effect of Dietary Digestible Protein Levels on Muscle Growth and Oxidative Stress in Amazonian Pintado (Pseudoplatystoma reticulatum × Leiarius marmoratus).

IF 3.6 3区 生物学 Q1 BIOLOGY
Stephane Vasconcelos Leandro, Daniel Rabello Ituassú, Valéria Dornelles Gindri Sinhorin, Danilo Henrique Aguiar, Paula Sueli Andrade Moreira, Ana Julia Lopes Braga Ferneda, Soraia Andressa Dall'Agnol Marques, Adilson Paulo Sinhorin, Anderson Corassa, Ana Paula Silva Ton, Leonardo Willian de Freitas, Maicon Sbardella
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Abstract

This study aimed to evaluate the effects of dietary digestible protein levels on the growth dynamics and oxidative stress status of white muscle fibers in Amazonian Pintado (Pseudoplatystoma reticulatum × Leiarius marmoratus). Four hundred and fifty-five juveniles of Amazonian Pintado were fed diets containing varying digestible protein levels (225, 250, 275, 300, 325, 350, or 375 g kg-1) for 75 days. At the end of the experiment, the fish were fasted for 24 h, anesthetized, and euthanized to obtain muscle samples. The linear and quadratic effects of dietary digestible protein levels on white muscle fiber diameter, metabolite concentrations, and oxidative stress were assessed. The results revealed that increasing dietary digestible protein levels linearly raised the concentrations of free amino acids and total proteins in muscle tissue but also led to elevated levels of TBARS, indicating increased oxidative stress. Notably, the average area of muscle fibers with a cell area greater than 1133 µm2 decreased, reflecting restricted muscle hypertrophy, whereas glycogen and glucose levels also declined. These findings suggest that although high dietary digestible protein enhances protein and free amino acid concentrations in muscle tissue, it may compromise muscle hypertrophy and increase oxidative damage in Amazonian Pintado, underscoring the complexity of optimizing diet formulation.

膳食可消化蛋白质水平对亚马孙平头鱼(Pseudoplatystoma reticulatum × Leiarius marmoratus)肌肉生长和氧化应激的影响。
本研究旨在评估日粮可消化蛋白质水平对亚马逊平头鱼(Pseudoplatystoma reticulatum × Leiarius marmoratus)生长动态和白色肌肉纤维氧化应激状态的影响。给四百五十五条亚马逊平头鱼幼鱼喂食含有不同可消化蛋白质水平(225、250、275、300、325、350 或 375 g kg-1)的饲料,为期 75 天。实验结束后,对鱼类禁食 24 小时,进行麻醉并安乐死,以获取肌肉样本。评估了日粮可消化蛋白质水平对白肌纤维直径、代谢物浓度和氧化应激的线性和二次效应。结果显示,日粮可消化蛋白质水平的增加会线性地提高肌肉组织中游离氨基酸和总蛋白质的浓度,但也会导致 TBARS 水平的升高,表明氧化应激增加。值得注意的是,细胞面积大于 1133 µm2 的肌肉纤维的平均面积减少,反映出肌肉肥大受到限制,而糖原和葡萄糖水平也有所下降。这些研究结果表明,虽然日粮中可消化蛋白质含量高会提高肌肉组织中蛋白质和游离氨基酸的浓度,但它可能会影响亚马逊平塔多的肌肉肥大并增加氧化损伤,这凸显了优化日粮配方的复杂性。
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来源期刊
Biology-Basel
Biology-Basel Biological Science-Biological Science
CiteScore
5.70
自引率
4.80%
发文量
1618
审稿时长
11 weeks
期刊介绍: Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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