A Nanoparticle-Coated Cellulose Acetate Membrane for Highly Efficient, Low-Cost Circulating Tumor Cell Detection.

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL
Yize Zhao, Yaqi Pan, Hao Sun, Pengfei Huo, Guangtong Wang, Shaoqin Liu
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Abstract

Detecting circulating tumor cells has exhibited great significance in treating cancers since its concentration is an index strongly associated with the development and transfer of the tumor. However, the present commercial method for CTC detection is still expensive, because special antibodies and complicated devices must be used for cell separation and imaging. Hence, it is quite necessary to apply alternative materials and methods to decrease the cost of CTC detection. In this article, we coated a cellulose acetate membrane with nanoparticles formed by the polymerization of melamine and furfural, creating a surface with nanoscale roughness for the highly efficient capture of the sparse CTCs in a blood sample. Subsequently, the CTCs on the surface can be quantitatively detected by colorimetry with the aid of a COF-based nanozyme. The detection limit (LOD) can be as low as 3 cells/mL, which is the lowest LOD among the colorimetric methods to our knowledge. Considering the low cost of fabricating the membrane for CTC capture and the robustness of nanozymes compared with natural enzymes, this CTC detection approach displays great potential to decrease the financial burden of commercial CTC detection.

一种用于高效、低成本检测循环肿瘤细胞的纳米颗粒包裹醋酸纤维素膜。
由于循环肿瘤细胞的浓度与肿瘤的发展和转移密切相关,因此检测循环肿瘤细胞对治疗癌症具有重要意义。然而,由于细胞分离和成像必须使用特殊的抗体和复杂的设备,目前商业化的 CTC 检测方法仍然价格昂贵。因此,应用其他材料和方法来降低 CTC 检测成本是非常必要的。在本文中,我们在醋酸纤维素膜上涂覆了由三聚氰胺和糠醛聚合而成的纳米颗粒,形成了具有纳米级粗糙度的表面,可高效捕获血液样本中稀疏的 CTC。随后,表面上的 CTC 可借助基于 COF 的纳米酶通过比色法进行定量检测。检测限(LOD)可低至 3 个细胞/毫升,这是我们所知的比色法中最低的检测限。考虑到用于捕获四氯化碳的膜的低成本制造以及纳米酶与天然酶相比的稳健性,这种四氯化碳检测方法在减轻商业四氯化碳检测的经济负担方面显示出巨大的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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