Paper-Based DNA Biosensor for Rapid and Selective Detection of miR-21.

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL
Alexander Hunt, Sri Ramulu Torati, Gymama Slaughter
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引用次数: 0

Abstract

Cancer is the second leading cause of death globally, with 9.7 million fatalities in 2022. While routine screenings are vital for early detection, healthcare disparities persist, highlighting the need for equitable solutions. Recent advancements in cancer biomarker identification, particularly microRNAs (miRs), have improved early detection. MiR-21 is notably overexpressed in various cancers and can be a valuable diagnostic tool. Traditional detection methods, though accurate, are costly and complex, limiting their use in resource-limited settings. Paper-based electrochemical biosensors offer a promising alternative, providing cost-effective, sensitive, and rapid diagnostics suitable for point-of-care use. This study introduces an innovative electrochemical paper-based biosensor that leverages gold inkjet printing for the quantitative detection of miR-21. The biosensor, aimed at developing cost-effective point-of-care devices for low-resource settings, uses thiolated self-assembled monolayers to immobilize single-stranded DNA-21 (ssDNA-21) on electrodeposited gold nanoparticles (AuNPs) on the printed gold surface, facilitating specific miR-21 capture. The hybridization of ssDNA-21 with miR-21 increases the anionic barrier density, impeding electron transfer from the redox probe and resulting in a current suppression that correlates with miR-21 concentration. The biosensor exhibited a linear detection range from 1 fM to 1 nM miR-21 with a sensitivity of 7.69 fM µA-1 cm2 and a rapid response time (15 min). With a low detection limit of 0.35 fM miR-21 in serum, the biosensor also demonstrates excellent selectivity against interferent species. This study introduces an electrochemical paper-based biosensor that uses gold inkjet printing to precisely detect miR-21, a key biomarker overexpressed in various cancers. This innovative device highlights the potential for cost-effective, accessible cancer diagnostics in underserved areas.

用于快速选择性检测 miR-21 的纸基 DNA 生物传感器
癌症是全球第二大死因,2022 年将有 970 万人死于癌症。虽然常规筛查对早期检测至关重要,但医疗保健方面的差距依然存在,这凸显了公平解决方案的必要性。癌症生物标志物鉴定方面的最新进展,尤其是微小核糖核酸(miRs),改善了早期检测。MiR-21 在各种癌症中明显过表达,可以成为一种有价值的诊断工具。传统的检测方法虽然准确,但成本高且复杂,限制了它们在资源有限的环境中的应用。基于纸张的电化学生物传感器提供了一种前景广阔的替代方法,可提供适合床旁使用的经济、灵敏、快速的诊断方法。本研究介绍了一种创新的电化学纸基生物传感器,它利用金喷墨打印技术对 miR-21 进行定量检测。该生物传感器旨在为低资源环境开发具有成本效益的床旁设备,它利用硫醇自组装单层将单链 DNA-21 (ssDNA-21)固定在印刷金表面的电沉积金纳米粒子(AuNPs)上,从而促进特异性 miR-21 捕获。ssDNA-21 与 miR-21 的杂交增加了阴离子阻挡密度,阻碍了氧化还原探针的电子传递,导致电流抑制,而电流抑制与 miR-21 的浓度相关。该生物传感器的线性检测范围为 1 fM 至 1 nM miR-21,灵敏度为 7.69 fM µA-1 cm2,响应时间短(15 分钟)。该生物传感器的检测限低至血清中的 0.35 fM miR-21,而且对干扰物具有极佳的选择性。本研究介绍了一种基于电化学纸的生物传感器,它利用金喷墨打印技术精确检测各种癌症中过度表达的关键生物标志物 miR-21。这种创新装置凸显了在服务欠缺地区进行经济、便捷的癌症诊断的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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