Effective Boundary Correction for Deterministic Lateral Displacement Microchannels to Improve Cell Separation: A Numerical and Experimental Study.

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL
Shaghayegh Mirhosseini, Mohammadmahdi Eskandarisani, Aryanaz Faghih Nasiri, Fatemeh Khatami, Akram Mirzaei, Majid Badieirostami, Seyed Mohammad Kazem Aghamir, Mohammadreza Kolahdouz
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Abstract

Particle separation and sorting techniques based on microfluidics have found extensive applications and are increasingly gaining prominence. This research presents the design and fabrication of a microfluidic device for separating cells using deterministic lateral displacement (DLD), enabling accuracy and continuity while being size-based. Nevertheless, it remains demanding, to completely reverse the detrimental effects of the boundaries that disturb the fluidic flow in the channel and reduce particle separation efficiency. This study introduces a novel approach to enhance the boundary structure of channels. By using this design, separation efficiency is boosted, and the fluid behavior around the walls is improved. The boundary correction (BC) enhances the operation of the microchannel and is very effective in microchannels. With boundary correction, the device exhibited improved separation efficiencies, but in its absence, separation efficiencies dropped. The collected microscopic images of the isolation of prostate cancer cell lines and red blood cells revealed promising outcomes. The efficiency of circulating tumor cell (CTC) throughput in the microfluidic channel, quantified as the ratio or proportion of tumor cells exiting the channel to cells entering it, exceeds 93%. Moreover, the efficiency of CTC isolation, expressed as the proportion of tumor cells from the upper outlet of the microfluidic channel to all cells, is over 89%. Additionally, the efficiency of red blood cell isolation, evaluated as the ratio of red blood cells from the lower outlet of the microfluidic channel to all cells, surpasses 77%. While using the same DLD separator without boundary correction reduced the separation efficiency by around 5%.

确定性侧向位移微通道的有效边界校正以改善细胞分离:数值和实验研究。
基于微流控技术的粒子分离和分拣技术已得到广泛应用,并日益受到重视。本研究介绍了一种利用确定性横向位移(DLD)分离细胞的微流体设备的设计与制造,该设备在基于尺寸的基础上实现了精确性和连续性。然而,要完全扭转边界的有害影响,使其扰乱通道中的流体流动并降低颗粒分离效率,仍然是一项艰巨的任务。本研究介绍了一种增强通道边界结构的新方法。通过使用这种设计,分离效率得到了提高,壁面周围的流体行为也得到了改善。边界校正(BC)增强了微通道的运行,在微通道中非常有效。有了边界校正,设备的分离效率得到提高,但如果没有边界校正,分离效率则会下降。收集到的前列腺癌细胞系和红细胞分离显微图像显示了良好的结果。循环肿瘤细胞(CTC)在微流体通道中的吞吐效率超过了 93%,其量化指标是流出通道的肿瘤细胞与进入通道的细胞的比率或比例。此外,以微流体通道上部出口的肿瘤细胞占所有细胞的比例表示的 CTC 分离效率超过 89%。此外,以来自微流体通道下部出口的红细胞与所有细胞的比例表示的红细胞分离效率也超过了 77%。而使用未进行边界校正的相同 DLD 分离器,分离效率降低了约 5%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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