Staphylococcus felis C4 exhibits in vitro antimicrobial activity against methicillin-resistant Staphylococcus pseudintermedius in a novel canine skin explant model.

IF 1.9 3区 农林科学 Q3 DERMATOLOGY
Mayane Faccin, Alan M O'Neill, Sara D Lawhon, Kate A Worthing, Dominique J Wiener, Richard L Gallo, Aline Rodrigues Hoffmann
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Abstract

Background: Canine superficial pyoderma is a common bacterial skin infection of dogs, generally caused by Staphylococcus pseudintermedius. The C4 strain of Staphylococcus felis was recently discovered to have strong antimicrobial activity against S. pseudintermedius in mice.

Objectives: We aimed to evaluate in vitro if this antimicrobial activity was maintained using a novel canine skin explant model.

Materials and methods: Punch biopsies (8 mm) of skin from recently euthanised dogs were collected and placed into six-well plates on top of an agarose pedestal.

Results: Histological examination of the skin explants showed an intact dermal-epidermal organisation and a stratum corneum that was successfully colonised by S. pseudintermedius after topical application. The number of colony forming units of S. pseudintermedius showed a 2 log increase after 24 h colonisation, indicating that the explant supported bacterial growth. By contrast, co-treatment with S. felis C4 live bacteria and its sterile protein product significantly reduced the growth of a methicillin-susceptible (ST540, p = 0.0357) and a methicillin-resistant (MR) strain (ST71, p = 0.0143) of S. pseudintermedius. No detectable bacteria were recovered from or visualised on skin 24 h posttreatment with the S. felis C4 sterile protein product.

Conclusions and clinical relevance: Using a novel canine explant model, we demonstrate that the S. felis C4 strain inhibits the growth of S. pseudintermedius and that it is a promising candidate for a new probiotic therapy to treat cutaneous infections caused by S. pseudintermedius, including MR strains.

猫科葡萄球菌 C4 在新型犬皮肤外植体模型中对耐甲氧西林伪中间葡萄球菌具有体外抗菌活性。
背景:犬浅表性脓皮病是一种常见的犬皮肤细菌感染,通常由假中间葡萄球菌引起。最近发现猫葡萄球菌的 C4 菌株对小鼠体内的假中间葡萄球菌有很强的抗菌活性:我们旨在使用一种新型犬皮肤切片模型,在体外评估这种抗菌活性是否得以维持:材料: 收集最近安乐死的狗的皮肤切片(8 毫米),并将其放入琼脂糖底座上的六孔板中:结果:对皮肤切片的组织学检查显示,真皮-表皮组织完整,角质层在局部应用后被假中间体成功定殖。假性角膜炎双球菌的菌落形成单位数在定植 24 小时后增加了 2 个对数,表明外植体支持细菌生长。相比之下,与 S. felis C4 活菌及其无菌蛋白产物共同处理可显著减少假中间芽孢杆菌的甲氧西林敏感菌株(ST540,p = 0.0357)和甲氧西林耐药(MR)菌株(ST71,p = 0.0143)的生长。使用猫科动物裂头蚴 C4 无菌蛋白产品处理 24 小时后,皮肤上未发现可检测到的细菌:通过使用一种新型犬外植体模型,我们证明了 S. felis C4 菌株可抑制伪中间肠球菌的生长,是治疗由伪中间肠球菌(包括 MR 菌株)引起的皮肤感染的新型益生菌疗法的理想候选菌株。
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来源期刊
Veterinary dermatology
Veterinary dermatology 农林科学-兽医学
CiteScore
3.20
自引率
21.40%
发文量
92
审稿时长
12-24 weeks
期刊介绍: Veterinary Dermatology is a bi-monthly, peer-reviewed, international journal which publishes papers on all aspects of the skin of mammals, birds, reptiles, amphibians and fish. Scientific research papers, clinical case reports and reviews covering the following aspects of dermatology will be considered for publication: -Skin structure (anatomy, histology, ultrastructure) -Skin function (physiology, biochemistry, pharmacology, immunology, genetics) -Skin microbiology and parasitology -Dermatopathology -Pathogenesis, diagnosis and treatment of skin diseases -New disease entities
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