Possible Causes of Extreme Variation of Benzo[a]pyrene Acute Toxicity Test on Daphnia magna.

IF 3.9 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Toxics Pub Date : 2024-09-30 DOI:10.3390/toxics12100714

Zi-Yi Zheng, Yu-Ting Yang, Jing-Xuan Zhou, Zhao-Xing Peng, Hong-Gang Ni

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引用次数: 0

Abstract

There are enormous differences in benzo[a]pyrene (BaP) acute toxicity tests on Daphnia magna, according to previous publications. The explanations of the reasons for this extreme variation are necessary. In this context, the acute toxicity tests of different experiment conditions (light/dark, culture medium, and solvent) were conducted on Daphnia magna with BaP as the toxicant of concern. Based on the experiments above, molecular dynamics (MD) simulations were employed to investigate the mechanisms of action. According to our results, the significant influence of light exposure on the acute toxicity test of BaP (p < 0.05) on D. magna was recorded. On the basis of the MD simulations, it was possible that BaP may not affect the normal operation of Superoxide Dismutase and Catalase directly, and it could be quickly transferred from the body through Glutathione S-transferase and Cytochromes P450. Therefore, when exposed to light, the oxidative stress process intensifies, causing damage to Daphnia magna. Apparently, the ecotoxicity tests based on inhibition for D. magna cannot adequately reflect the toxic effects of BaP.

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苯并[a]芘对大型蚤急性毒性试验极端变异的可能原因。

根据以往的出版物，苯并[a]芘（BaP）对大型蚤的急性毒性试验存在巨大差异。有必要解释这种极端差异的原因。为此，我们以 BaP 为毒性物质，对大型蚤进行了不同实验条件（光照/黑暗、培养基和溶剂）下的急性毒性试验。在上述实验的基础上，采用分子动力学（MD）模拟来研究其作用机制。结果表明，光照对 BaP 对大型蚤的急性毒性试验有显著影响（p < 0.05）。在 MD 模拟的基础上，BaP 可能不会直接影响超氧化物歧化酶和过氧化氢酶的正常工作，而是通过谷胱甘肽 S 转移酶和细胞色素 P450 迅速转移出体外。因此，在光照下，氧化应激过程会加剧，对大型蚤造成损害。显然，基于对大型蚤抑制作用的生态毒性测试不能充分反映 BaP 的毒性作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxics Chemical Engineering-Chemical Health and Safety

CiteScore

4.50

自引率

10.90%

发文量

681

审稿时长

6 weeks

期刊介绍： The Journal accepts papers describing work that furthers our understanding of the exposure, effects, and risks of chemicals and materials in humans and the natural environment as well as approaches to assess and/or manage the toxicological and ecotoxicological risks of chemicals and materials. The journal covers a wide range of toxic substances, including metals, pesticides, pharmaceuticals, biocides, nanomaterials, and polymers such as micro- and mesoplastics. Toxics accepts papers covering: The occurrence, transport, and fate of chemicals and materials in different systems (e.g., food, air, water, soil); Exposure of humans and the environment to toxic chemicals and materials as well as modelling and experimental approaches for characterizing the exposure in, e.g., water, air, soil, food, and consumer products; Uptake, metabolism, and effects of chemicals and materials in a wide range of systems including in-vitro toxicological assays, aquatic and terrestrial organisms and ecosystems, model mammalian systems, and humans; Approaches to assess the risks of chemicals and materials to humans and the environment; Methodologies to eliminate or reduce the exposure of humans and the environment to toxic chemicals and materials.