Both GacS-regulated lipopeptides and the type three secretion system contribute to Pseudomonas cichorii induced necrosis in lettuce and chicory.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Chien-Jui Huang, Ellen Pauwelyn, Marc Ongena, Peter Bleyaert, Monica Höfte
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Abstract

Pseudomonas cichorii SF1-54, the causal agent of lettuce midrib rot disease, produces lipopeptides cichofactins and cichopeptins which are important virulence factors. The GacS/GacA two-component system is well known to regulate production of lipopeptides in pseudomonads. Additionally, the functions of the type three secretion system (T3SS) in P. cichorii-plant interactions are not clarified. In this study, we investigated the role of the GacS-regulated lipopeptides and the T3SS in pathogenicity of P. cichorii SF1-54 on two host plants, chicory and lettuce, by constructing mutants in hrpL, which encodes the key sigma factor to control T3SS expression, and gacS. Compared with the wildtype, the hrpL mutant produced lipopeptides at a similar level but the gacS mutant was strongly impaired in lipopeptide production. The mutant deficient in hrpL did not significantly differ from the wildtype in virulence on chicory and lettuce. The gacS mutant exhibited significantly less symptoms on both host plants compared to the wildtype and the hrpL mutant. Intriguingly, the gacS hrpL-double mutant no longer produced lipopeptides, lost virulence and showed impaired colonization on chicory, but was still weakly virulent on lettuce. Thus, contribution of both the GacS-regulated lipopeptides and T3SS to virulence of P. cichorii SF1-54 is host plant dependent.

GacS调控的脂肽和三型分泌系统都有助于cichorii假单胞菌诱导莴苣和菊苣坏死。
莴苣中肋腐烂病的病原菌 Pseudomonas cichorii SF1-54 能产生脂肽 cichofactins 和 cichopeptins,它们是重要的毒力因子。众所周知,GacS/GacA 双组分系统可以调节假单胞菌脂肽的产生。此外,三型分泌系统(T3SS)在 P. cichorii- 植物相互作用中的功能尚未明确。在本研究中,我们通过构建编码控制 T3SS 表达的关键 sigma 因子 hrpL 和 gacS 的突变体,研究了 GacS 调控的脂肽和 T3SS 在 P. cichorii SF1-54 对菊苣和莴苣两种寄主植物的致病性中的作用。与野生型相比,hrpL 突变体产生脂肽的水平相似,但 gacS 突变体产生脂肽的能力严重受损。缺乏 hrpL 的突变体对菊苣和莴苣的毒力与野生型没有显著差异。与野生型和 hrpL 突变体相比,gacS 突变体在两种寄主植物上表现出的症状明显较少。耐人寻味的是,gacS hrpL 双突变体不再产生脂肽,失去了毒力,在菊苣上的定殖能力受损,但在莴苣上仍有弱毒力。因此,GacS 调控的脂肽和 T3SS 对 P. cichorii SF1-54 的毒力都有依赖性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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