Fabrication and Properties of Hydrogel Dressings Based on Genipin Crosslinked Chondroitin Sulfate and Chitosan.

IF 4.7 3区工程技术 Q1 POLYMER SCIENCE

Polymers Pub Date : 2024-10-11 DOI:10.3390/polym16202876

Ling Wang, Xiaoyue Ding, Xiaorui He, Ning Tian, Peng Ding, Wei Guo, Oseweuba Valentine Okoro, Yanfang Sun, Guohua Jiang, Zhenzhong Liu, Armin Shavandi, Lei Nie

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引用次数: 0

Abstract

Multifunctional hydrogel dressings remain highly sought after for the promotion of skin wound regeneration. In the present study, multifunctional CHS-DA/HACC (CH) hydrogels with an interpenetrated network were constructed using hydroxypropyl trimethyl ammonium chloride modified chitosan (HACC) and dopamine-modified chondroitin sulfate (CHS-DA), using genipin as crosslinker. The synthesis of HACC and CHS-DA was effectively confirmed using Fourier transform infrared (FT-IR) analysis and ¹H nuclear magnetic resonance (¹H NMR) spectroscopy. The prepared CH hydrogels exhibited a network of interconnected pores within the microstructure. Furthermore, rheological testing demonstrated that CH hydrogels exhibited strong mechanical properties, stability, and injectability. Further characterization investigations showed that the CH hydrogels showed favorable self-healing and self-adhesion properties. It was also shown that increasing HACC concentration ratio was positively correlated with the antibacterial activity of CH hydrogels, as evidenced by their resistance to Escherichia coli and Staphylococcus aureus. Additionally, Cell Counting Kit-8 (CCK-8) tests, fluorescent images, and a cell scratch assay demonstrated that CH hydrogels had good biocompatibility and cell migration ability. The multifunctional interpenetrated network hydrogels were shown to have good antibacterial properties, antioxidant properties, stable storage modulus and loss modulus, injectable properties, self-healing properties, and biocompatibility, highlighting their potential as wound dressings in wound healing applications.

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基于吉尼平交联硫酸软骨素和壳聚糖的水凝胶敷料的制造与性能

多功能水凝胶敷料在促进皮肤伤口再生方面一直备受追捧。本研究以羟丙基三甲基氯化铵改性壳聚糖（HACC）和多巴胺改性硫酸软骨素（CHS-DA）为交联剂，以基因平为交联剂，构建了具有互穿网络的多功能 CHS-DA/HACC (CH) 水凝胶。傅立叶变换红外光谱（FT-IR）分析和 1H 核磁共振光谱（1H NMR）分析有效地证实了 HACC 和 CHS-DA 的合成。制备的 CH 水凝胶在微观结构中呈现出相互连接的孔隙网络。此外，流变测试表明 CH 水凝胶具有很强的机械性能、稳定性和可注射性。进一步的表征研究表明，CH 水凝胶具有良好的自愈合和自粘附性能。研究还表明，HACC 浓度比的增加与 CH 水凝胶的抗菌活性呈正相关，这体现在它们对大肠杆菌和金黄色葡萄球菌的抗性上。此外，细胞计数试剂盒-8（CCK-8）测试、荧光图像和细胞划痕试验都证明 CH 水凝胶具有良好的生物相容性和细胞迁移能力。研究表明，多功能互穿网络水凝胶具有良好的抗菌性、抗氧化性、稳定的储存模量和损耗模量、可注射性、自愈合性和生物相容性，突出了其在伤口愈合应用中作为伤口敷料的潜力。

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来源期刊

Polymers POLYMER SCIENCE-

CiteScore

8.00

自引率

16.00%

发文量

4697

审稿时长

1.3 months

期刊介绍： Polymers (ISSN 2073-4360) is an international, open access journal of polymer science. It publishes research papers, short communications and review papers. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Polymers provides an interdisciplinary forum for publishing papers which advance the fields of (i) polymerization methods, (ii) theory, simulation, and modeling, (iii) understanding of new physical phenomena, (iv) advances in characterization techniques, and (v) harnessing of self-assembly and biological strategies for producing complex multifunctional structures.