Genome-wide identification of HIPP and mechanism of SlHIPP4/7/9/21/26/32 mediated phytohormones response to Cd, osmotic, and salt stresses in tomato

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-10-19 DOI:10.1016/j.plaphy.2024.109220

Junrong Xu , Jing Cui , Qiuyu He , Yunzhi Liu , Xuefang Lu , Jin Qi , Jingli Xiong , Wenjin Yu , Changxia Li

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引用次数: 0

Abstract

Heavy-metal-associated isoprenylated plant proteins (HIPPs) contributed to abiotic tolerance in vascular plants. Up to now, the HIPP gene family of tomato (Solanum lycopersicum L.) had not been thoroughly understood. In the present study, 34 SlHIPP genes were identified from the tomato genome using the Hidden Markov Model (HMM). The phylogenetic analysis revealed that the evolution of SlHIPPs was highly conserved. The cis-acting element analysis indicated that SlHIPP genes might be involved in phytohormones and abiotic stresses. We constructed venn diagram with 17 genes containing stress-related motifs as well as 15 genes and 19 genes expressing in leaves and roots in RNA-seq data, suggesting that SlHIPP4/7/9/21/26/32 were selected as candidate genes for study. The quantitative real-time PCR (qRT-PCR) analysis showed that 6 candidate genes were indicated to be involved in osmotic and salt stress tolerance and SlHIPP7/21/26/32 responded to cadmium (Cd) tolerance. The virus-induced silencing of 6 candidate genes caused growth inhibition in stress conditions, further illustrating that 6 candidate genes played a positive role in abiotic conditions. Importantly, the phytohormone analysis implied that 6 candidate genes mediated abscisic acid (ABA), salicylic acid (SA), gibberellin (GA₃), auxin (IAA), or methyl jasmonate (MeJA) response to Cd, osmotic, or salt stress tolerance. These findings indicated that SlHIPP4/7/9/21/26/32 were key regulators of abiotic stress responses in tomato seedlings, functioning through multiple phytohormone pathways.

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全基因组范围内鉴定番茄的 HIPP 以及 SlHIPP4/7/9/21/26/32 介导的植物激素对镉、渗透压和盐胁迫的响应机制。

重金属相关异戊烯化植物蛋白（HIPPs）有助于维管束植物的抗逆性。迄今为止，人们对番茄（Solanum lycopersicum L.）的 HIPP 基因家族还没有透彻的了解。本研究利用隐马尔可夫模型（HMM）从番茄基因组中鉴定了 34 个 SlHIPP 基因。系统进化分析表明，SlHIPPs 的进化高度保守。顺式作用元件分析表明，SlHIPP基因可能与植物激素和非生物胁迫有关。我们构建了17个含有胁迫相关基因的维恩图，以及RNA-seq数据中在叶和根表达的15个基因和19个基因，表明SlHIPP4/7/9/21/26/32被选为候选研究基因。实时定量 PCR（qRT-PCR）分析表明，6 个候选基因参与了渗透胁迫和盐胁迫的耐受，而 SlHIPP7/21/26/32 则对镉（Cd）的耐受有反应。病毒诱导的 6 个候选基因沉默会导致胁迫条件下的生长抑制，进一步说明 6 个候选基因在非生物条件下发挥了积极作用。重要的是，植物激素分析表明，6 个候选基因介导了脱落酸（ABA）、水杨酸（SA）、赤霉素（GA3）、辅助素（IAA）或茉莉酸甲酯（MeJA）对镉、渗透压或盐胁迫耐受性的响应。这些发现表明，SlHIPP4/7/9/21/26/32是番茄幼苗非生物胁迫反应的关键调控因子，通过多种植物激素途径发挥作用。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.