Contribution of the regulatory miR156-SPL9 module to the drought stress response in pigmented potato (Solanum tuberosum L.)

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-10-12 DOI:10.1016/j.plaphy.2024.109195

Sara Pescador-Dionisio , Aida Robles-Fort , Bruno Parisi , Inmaculada García-Robles , Laura Bassolino , Giuseppe Mandolino , M. Dolores Real , Carolina Rausell

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引用次数: 0

Abstract

Potato (Solanum tuberosum L.) is nowadays an important component of diversified cropping systems due to its adaptability, yielding capacity, and nutrition contribution. Breeding programs aiming at raising potato's nutritional value have mainly focused on the accumulation in potato tubers of health-promoting phytochemicals such as anthocyanins. In different plant species, increased amounts of anthocyanins in vegetative tissues have been associated with enhanced tolerance to abiotic and biotic stresses that challenge agrifood systems in the current context of global climate change. In the present study, we aimed at gaining insight into the effect of anthocyanin accumulation on the potato plants response to drought stress using three different potato genotypes with differential canopy and tuber pigmentation: the purple fleshed commercial variety Bleuet; the red fleshed breeding clone DAR170; and the non-pigmented commercial variety Monalisa. The varieties Bleuet and DAR170 exhibiting higher anthocyanin content in vegetative tissues than the Monalisa variety showed a remarkable inhibition of stem growth development under drought stress treatment suggestive of an anthocyanin-mediated physiological shift from growth to resilience as a mechanism of stress tolerance. The results of the expression analysis of stu-miR156a and its target StSPL9 gene in the potato plants with different anthocyanin content, as well as their change in response to drought stress support the participation of the conserved miR156-SPL9 regulatory module in coordinating potato plants development and plant responses to drought stress, involving precise fine-tuning of anthocyanin biosynthesis.

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调控 miR156-SPL9 模块对色素马铃薯（Solanum tuberosum L.）干旱胁迫响应的贡献

马铃薯（Solanum tuberosum L.）因其适应性强、产量高、营养丰富，如今已成为多样化种植系统的重要组成部分。旨在提高马铃薯营养价值的育种计划主要侧重于在马铃薯块茎中积累花青素等促进健康的植物化学物质。在不同的植物物种中，无性组织中花青素含量的增加与对非生物和生物胁迫的耐受性增强有关，而在当前全球气候变化的背景下，这些胁迫对农业食品系统提出了挑战。在本研究中，我们使用了三种不同的马铃薯基因型，它们的冠层和块茎色素不同，分别是紫色肉质的商业品种Bleuet、红色肉质的育种克隆DAR170和无色素的商业品种Monalisa，旨在深入了解花青素积累对马铃薯植株应对干旱胁迫的影响。与 Monalisa 品种相比，Bleuet 和 DAR170 在无性组织中的花青素含量更高，在干旱胁迫处理下显著抑制了茎的生长发育，这表明花青素介导的从生长到恢复的生理转变是一种胁迫耐受机制。stu-miR156a及其靶标StSPL9基因在不同花青素含量的马铃薯植株中的表达分析结果及其对干旱胁迫的响应变化支持了保守的miR156-SPL9调控模块参与协调马铃薯植株的发育和植株对干旱胁迫的响应，涉及花青素生物合成的精确微调。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.