Laoxianghuang polysaccharide promotes the anti-inflammatory cytokine interleukin-10 in colitis via gut microbial linoleic acid.

IF 6.7 1区医学 Q1 CHEMISTRY, MEDICINAL

Phytomedicine Pub Date : 2024-10-09 DOI:10.1016/j.phymed.2024.156136

Yuwei Zhang, Yiren Yang, Jiangping Song, Wenqing Yu, Yaqian Li, Denghong Liu, Jie Gao, Bei Fan, Fengzhong Wang, Yang Zheng

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引用次数: 0

Abstract

Background: Our previous study found that the polysaccharide from Laoxianghuang (LP), fermented fruit of bergamot (traditional Chinese medicine and food), can alter gut microbiota and regulate short-chain fatty acids (SCFAs) in vitro. Nevertheless, there is a paucity of reports on the impact of LP on gut microbiota in vivo.

Purpose: To analyze the structures of LP, investigate the influence of LP on the damaged intestinal barrier in DSS-induced colitis mice, and further explore its potential mechanisms.

Methods: We analyzed the physicochemical properties of purified LP by HPLC, SEM, and FT-IR spectrum. Then, to assess the effect of LP in DSS-induced colitis mice, we observed the damage to the colon tissue, measured inflammatory cytokines and tight junction protein expression through RT-qPCR as well as immunofluorescent staining, and investigated the influence of LP on altering gut microbiota and metabolites using 16 s rRNA sequencing and HPLC-MS/MS. Ultimately, the impact of linoleic acid on inflammatory cytokines was confirmed by the LPS-induced RAW264.7 cells.

Results: LP, mainly galactoglucan, could inhibit weight loss and colon shortening, decrease levels of tumor necrosis factor-α (TNF-α), increase levels of interleukin-10 (IL-10) and the intestinal acetic acid and butyric acid, and promote the expression of tight junction proteins ZO-1 and Claudin-1. Meanwhile, LP enhanced the abundance of beneficial bacteria including Romboutsia, Eubacterium_coprostanoligenes_group, and Akkermansia, and regulated linoleic acid metabolism to increase the linoleic acid level. In vitro cell experiment proved that linoleic acid could elevate the level of IL-10 and inhibit inflammatory responses.

Conclusions: Our results suggested that LP effectively alleviated colitis by promoting the anti-inflammatory cytokine interleukin-10 via gut microbiota-mediated linoleic acid metabolism.

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老香黄多糖通过肠道微生物亚油酸促进结肠炎抗炎细胞因子白细胞介素-10的生成

背景：我们之前的研究发现，佛手柑（中药和食品）发酵果实老香黄多糖（Laoxianghuang，LP）能在体外改变肠道微生物群并调节短链脂肪酸（SCFAs）。目的：分析佛手柑的结构，研究佛手柑对 DSS 诱导的结肠炎小鼠肠道屏障受损的影响，并进一步探讨其潜在机制：方法：通过高效液相色谱、扫描电镜和傅立叶变换红外光谱分析纯化LP的理化性质。然后，为了评估亚麻油对DSS诱导的结肠炎小鼠的影响，我们观察了结肠组织的损伤情况，通过RT-qPCR和免疫荧光染色测定了炎性细胞因子和紧密连接蛋白的表达，并利用16 s rRNA测序和HPLC-MS/MS研究了亚麻油对肠道微生物群和代谢物改变的影响。最后，通过 LPS 诱导的 RAW264.7 细胞证实了亚油酸对炎症细胞因子的影响：结果：以半乳聚糖为主的亚油酸能抑制体重下降和结肠缩短，降低肿瘤坏死因子-α（TNF-α）的水平，提高白细胞介素-10（IL-10）和肠道乙酸、丁酸的水平，促进紧密连接蛋白ZO-1和Claudin-1的表达。同时，LP 还能提高有益菌（包括 Romboutsia、Eubacterium_coprostanoligenes_group 和 Akkermansia）的数量，并调节亚油酸代谢，提高亚油酸水平。体外细胞实验证明，亚油酸能提高 IL-10 的水平，抑制炎症反应：我们的研究结果表明，LP 可通过肠道微生物群介导的亚油酸代谢促进抗炎细胞因子白细胞介素-10 的产生，从而有效缓解结肠炎。

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来源期刊

Phytomedicine 医学-药学

CiteScore

10.30

自引率

5.10%

发文量

670

审稿时长

91 days

期刊介绍： Phytomedicine is a therapy-oriented journal that publishes innovative studies on the efficacy, safety, quality, and mechanisms of action of specified plant extracts, phytopharmaceuticals, and their isolated constituents. This includes clinical, pharmacological, pharmacokinetic, and toxicological studies of herbal medicinal products, preparations, and purified compounds with defined and consistent quality, ensuring reproducible pharmacological activity. Founded in 1994, Phytomedicine aims to focus and stimulate research in this field and establish internationally accepted scientific standards for pharmacological studies, proof of clinical efficacy, and safety of phytomedicines.