Root exudation patterns of contrasting rice (Oryza sativa L.) lines in response to P limitation.

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2024-10-23 DOI:10.1007/s00425-024-04556-2
Henning Schwalm, Christiana Staudinger, Mohammad-Reza Hajirezaei, Eva Mundschenk, Alireza Golestanifard, Maire Holz, Matthias Wissuwa, Eva Oburger
{"title":"Root exudation patterns of contrasting rice (Oryza sativa L.) lines in response to P limitation.","authors":"Henning Schwalm, Christiana Staudinger, Mohammad-Reza Hajirezaei, Eva Mundschenk, Alireza Golestanifard, Maire Holz, Matthias Wissuwa, Eva Oburger","doi":"10.1007/s00425-024-04556-2","DOIUrl":null,"url":null,"abstract":"<p><strong>Main conclusion: </strong>Rice exudation patterns changed in response to P deficiency. Higher exudation rates were associated with lower biomass production. Total carboxylate exudation rates mostly decreased under P-limiting conditions. Within the rhizosphere, root exudates are believed to play an important role in plant phosphorus (P) acquisition. This could be particularly beneficial in upland rice production where P is often limited. However, knowledge gaps remain on how P deficiency shapes quality and quantity of root exudation in upland rice genotypes. We therefore investigated growth, plant P uptake, and root exudation patterns of two rice genotypes differing in P efficiency in semi-hydroponics at two P levels (low P = 1 µM, adequate P = 100 µM). Root exudates were collected hydroponically 28 and 40 days after germination to analyze total carbon (C), carbohydrates, amino acids, phenolic compounds spectrophotometrically and carboxylates using a targeted LC-MS approach. Despite their reported role in P solubilization, we observed that carboxylate exudation rates per unit root surface area were not increased under P deficiency. In contrast, exudation rates of total C, carbohydrates, amino acids and phenolics were mostly enhanced in response to low P supply. Overall, higher exudation rates were associated with lower biomass production in the P-inefficient genotype Nerica4, whereas the larger root system with lower C investment (per unit root surface area) in root exudates of the P-efficient DJ123 allowed for better plant growth under P deficiency. Our results reveal new insights into genotype-specific resource allocation in rice under P-limiting conditions that warrant follow-up research including more genotypes.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"260 6","pages":"123"},"PeriodicalIF":3.6000,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11499414/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Planta","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00425-024-04556-2","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Main conclusion: Rice exudation patterns changed in response to P deficiency. Higher exudation rates were associated with lower biomass production. Total carboxylate exudation rates mostly decreased under P-limiting conditions. Within the rhizosphere, root exudates are believed to play an important role in plant phosphorus (P) acquisition. This could be particularly beneficial in upland rice production where P is often limited. However, knowledge gaps remain on how P deficiency shapes quality and quantity of root exudation in upland rice genotypes. We therefore investigated growth, plant P uptake, and root exudation patterns of two rice genotypes differing in P efficiency in semi-hydroponics at two P levels (low P = 1 µM, adequate P = 100 µM). Root exudates were collected hydroponically 28 and 40 days after germination to analyze total carbon (C), carbohydrates, amino acids, phenolic compounds spectrophotometrically and carboxylates using a targeted LC-MS approach. Despite their reported role in P solubilization, we observed that carboxylate exudation rates per unit root surface area were not increased under P deficiency. In contrast, exudation rates of total C, carbohydrates, amino acids and phenolics were mostly enhanced in response to low P supply. Overall, higher exudation rates were associated with lower biomass production in the P-inefficient genotype Nerica4, whereas the larger root system with lower C investment (per unit root surface area) in root exudates of the P-efficient DJ123 allowed for better plant growth under P deficiency. Our results reveal new insights into genotype-specific resource allocation in rice under P-limiting conditions that warrant follow-up research including more genotypes.

水稻(Oryza sativa L.)不同品系的根系渗出模式对 P 限制的响应。
主要结论水稻的渗出模式随缺钾而改变。较高的渗出率与较低的生物量产量有关。在磷限制条件下,羧酸盐总渗出率大多下降。在根瘤层中,根系渗出物被认为在植物获取磷(P)方面发挥着重要作用。这对通常受磷限制的高地水稻生产尤其有益。然而,关于缺磷如何影响高地水稻基因型根系渗出物的质量和数量的知识仍然存在空白。因此,我们研究了在两种钾水平(低钾 = 1 µM,充足钾 = 100 µM)的半水培条件下,两种钾效率不同的水稻基因型的生长、植物钾吸收和根系渗出模式。发芽后 28 天和 40 天,通过水培收集根系渗出物,采用目标 LC-MS 方法分析总碳(C)、碳水化合物、氨基酸、酚类化合物的分光光度法和羧酸盐。尽管有报道称羧酸盐在钾溶解中发挥作用,但我们观察到,在缺钾情况下,单位根表面积的羧酸盐渗出率并没有增加。与此相反,总碳、碳水化合物、氨基酸和酚类物质的渗出率大多在低钾供应条件下有所提高。总体而言,钾效率低的基因型 Nerica4 的较高渗出率与较低的生物量产量有关,而钾效率高的 DJ123 根系较大,根系渗出物中的碳投资(单位根表面积)较低,因此在缺钾条件下植物生长较好。我们的研究结果揭示了水稻在钾限制条件下基因型特异性资源分配的新见解,值得包括更多基因型在内的后续研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信