Antimicrobial Activities of Essential Oils of Different Pinus Species from Bosnia and Herzegovina.

IF 4.9 3区医学 Q1 PHARMACOLOGY & PHARMACY

Pharmaceutics Pub Date : 2024-10-15 DOI:10.3390/pharmaceutics16101331

Snježana Mirković, Vanja Tadić, Marina T Milenković, Dušan Ušjak, Gordana Racić, Dragica Bojović, Ana Žugić

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引用次数: 0

Abstract

Background/objectives: The emergence of antimicrobial resistance has urged researchers to explore new antimicrobial agents, such as essential oils (EOs). The aim of this study was to examine chemical composition and antimicrobial activity of the EOs from the needles and green cones of four Pinus species (Pinus mugo Turra., P. nigra J.F., P. syilvestris L., and P. halepensis Miller) from Bosnia and Herzegovina.

Methods: Chemical profiles of EOs were assessed by gas chromatography, while microdilution method was used to test their antimicrobial activity. A synergistic action of EOs and gentamicin was investigated by the checkerboard assay.

Results: The chemical composition of the tested EOs showed a high percentage of α-pinene, (E)-caryophyllene, limonene, germacrene D, myrcene, and δ-3-carene. EO from green cones of P. sylvestris showed high efficiency against S. aureus and E. faecalis. The MIC of P. nigra cones' EO was 100 μg/mL against E. coli. The EO of P. halepensis green cones demonstrated the strongest activity against E. faecalis. EOs of P. halepensis needles and green cones exhibited the highest activity against C. albicans. Further, synergistic interaction was detected in combination of the selected EOs/gentamicin toward S. aureus and K. pneumoniae.

Conclusions: Among the tested EOs, oils of P. sylvestris cones and P. halepensis cones and needles showed the greatest antimicrobial activity. The same EOs and EO from P. nigra cones displayed synergistic potential in combination with gentamicin, supporting their utilization as antimicrobial agents alone or in combination with antibiotics, which is in line with their ethnopharmacological usage and circular bioeconomy principles.

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波斯尼亚和黑塞哥维那不同松树品种精油的抗菌活性。

背景/目的：抗菌剂耐药性的出现促使研究人员探索新的抗菌剂，如精油（EOs）。本研究旨在研究波斯尼亚和黑塞哥维那四种松树（Pinus mugo Turra.、P. nigra J.F.、P. syilvestris L.和 P. halepensis Miller）针叶和绿色球果中的 EO 的化学成分和抗菌活性：方法：采用气相色谱法评估 EO 的化学成分，并使用微量稀释法检测其抗菌活性。通过棋盘试验研究了 EO 和庆大霉素的协同作用：结果：测试的环氧乙烷的化学成分显示，α-蒎烯、（E）-石竹烯、柠檬烯、锗烯 D、月桂烯和δ-3-蒈烯的比例较高。西洋接骨木绿锥花的环氧乙烷对金黄色葡萄球菌和粪大肠杆菌具有很高的抗菌效率。黑锥栗环氧乙烷对大肠杆菌的 MIC 值为 100 μg/mL。P. halepensis 绿锥花的环氧乙烷对粪大肠杆菌的活性最强。P. halepensis 针叶和绿锥花的环氧乙烷对白僵菌的活性最高。此外，所选环氧乙烷/庆大霉素的组合对金黄色葡萄球菌和肺炎双球菌具有协同作用：结论：在所测试的环氧乙烷中，西洋鸢尾科植物圆锥花和半枝莲科植物圆锥花和针叶的油显示出最强的抗菌活性。同样的环氧乙烷和黑叶松果油在与庆大霉素联合使用时显示出协同潜力，支持将它们单独或与抗生素联合用作抗菌剂，这符合它们的民族药理学用途和循环生物经济原则。

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来源期刊

Pharmaceutics Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

7.90

自引率

11.10%

发文量

2379

审稿时长

16.41 days

期刊介绍： Pharmaceutics (ISSN 1999-4923) is an open access journal which provides an advanced forum for the science and technology of pharmaceutics and biopharmaceutics. It publishes reviews, regular research papers, communications, and short notes. Covered topics include pharmacokinetics, toxicokinetics, pharmacodynamics, pharmacogenetics and pharmacogenomics, and pharmaceutical formulation. Our aim is to encourage scientists to publish their experimental and theoretical details in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.