Molecular Identification and Bioinformatics Analysis of Anaplasma marginale Moonlighting Proteins as Possible Antigenic Targets.

IF 3.3 3区医学 Q2 MICROBIOLOGY

Pathogens Pub Date : 2024-09-28 DOI:10.3390/pathogens13100845

Rosa Estela Quiroz-Castañeda, Hugo Aguilar-Díaz, Eduardo Coronado-Villanueva, Diego Israel Catalán-Ochoa, Itzel Amaro-Estrada

{"title":"Molecular Identification and Bioinformatics Analysis of Anaplasma marginale Moonlighting Proteins as Possible Antigenic Targets.","authors":"Rosa Estela Quiroz-Castañeda, Hugo Aguilar-Díaz, Eduardo Coronado-Villanueva, Diego Israel Catalán-Ochoa, Itzel Amaro-Estrada","doi":"10.3390/pathogens13100845","DOIUrl":null,"url":null,"abstract":"Background: Diseases of veterinary importance, such as bovine Anaplasmosis, cause significant economic losses. Due to this, the study of various proteins of the causal agent Anaplasma marginale has focused on surface proteins. However, a vaccine for this disease is not yet available. To this end, in this work, moonlighting proteins (MLPs) are presented as an alternative approach for the design of immunogens against A. marginale.Methods: The proteins of the strain MEX-15-099-01 were analyzed, and its MLPs were identified. Subsequently, four virulence-associated MLP genes were selected and identified using PCR. The proteins were analyzed using a structural homology approach and the collection of B-cell epitopes was predicted for each MLP. Finally, a pair of AmEno peptides were synthesized and the antigenic potential was tested using an iELISA.Results: Our bioinformatics analysis revealed the potential of AmEno, AmGroEl, AmEF-Tu, and AmDnaK proteins as promising candidates for designing immunogens. The PCR allowed the gene sequence identification in the genome of the strain MEX-15-099-01. Notably, AmEno-derived synthetic peptides showed antigenicity in an ELISA.Conclusions: Our study has shed light on the potential use of MLPs for immunogen design, demonstrating the antigenic potential of AmEno.","PeriodicalId":19758,"journal":{"name":"Pathogens","volume":null,"pages":null},"PeriodicalIF":3.3000,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510912/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pathogens","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/pathogens13100845","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Diseases of veterinary importance, such as bovine Anaplasmosis, cause significant economic losses. Due to this, the study of various proteins of the causal agent Anaplasma marginale has focused on surface proteins. However, a vaccine for this disease is not yet available. To this end, in this work, moonlighting proteins (MLPs) are presented as an alternative approach for the design of immunogens against A. marginale.

Methods: The proteins of the strain MEX-15-099-01 were analyzed, and its MLPs were identified. Subsequently, four virulence-associated MLP genes were selected and identified using PCR. The proteins were analyzed using a structural homology approach and the collection of B-cell epitopes was predicted for each MLP. Finally, a pair of AmEno peptides were synthesized and the antigenic potential was tested using an iELISA.

Results: Our bioinformatics analysis revealed the potential of AmEno, AmGroEl, AmEF-Tu, and AmDnaK proteins as promising candidates for designing immunogens. The PCR allowed the gene sequence identification in the genome of the strain MEX-15-099-01. Notably, AmEno-derived synthetic peptides showed antigenicity in an ELISA.

Conclusions: Our study has shed light on the potential use of MLPs for immunogen design, demonstrating the antigenic potential of AmEno.

查看原文本刊更多论文

作为可能抗原靶标的边缘疟原虫月光蛋白的分子鉴定和生物信息学分析。

背景：对兽医具有重要意义的疾病，如牛无形体病，会造成巨大的经济损失。因此，对病原体边缘无形体（Anaplasma marginale）的各种蛋白质的研究主要集中在表面蛋白质上。然而，目前还没有针对这种疾病的疫苗。为此，本研究提出了月光蛋白（MLPs）作为设计边缘疟原虫免疫原的替代方法：方法：分析了 MEX-15-099-01 菌株的蛋白质，并确定了其 MLPs。随后，利用聚合酶链反应筛选并鉴定了四个与毒力相关的 MLP 基因。使用结构同源性方法对蛋白质进行了分析，并预测了每种 MLP 的 B 细胞表位集合。最后，合成了一对 AmEno 肽，并使用 iELISA 检测了其抗原潜力：结果：我们的生物信息学分析表明，AmEno、AmGroEl、AmEF-Tu 和 AmDnaK 蛋白具有设计免疫原的潜力。通过聚合酶链式反应（PCR），在菌株MEX-15-099-01的基因组中鉴定出了基因序列。值得注意的是，AmEno衍生的合成肽在ELISA中显示出抗原性：我们的研究揭示了 MLPs 在免疫原设计中的潜在用途，证明了 AmEno 的抗原潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Pathogens Medicine-Immunology and Allergy

CiteScore

6.40

自引率

8.10%

发文量

1285

审稿时长

17.75 days

期刊介绍： Pathogens (ISSN 2076-0817) publishes reviews, regular research papers and short notes on all aspects of pathogens and pathogen-host interactions. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.