Effects of bile acids on the growth, composition and metabolism of gut bacteria.

IF 7.8 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

npj Biofilms and Microbiomes Pub Date : 2024-10-23 DOI:10.1038/s41522-024-00566-w

Yi-Lei Peng, Si-Han Wang, Yu-Long Zhang, Man-Yun Chen, Kang He, Qing Li, Wei-Hua Huang, Wei Zhang

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Abstract

Bile acids (BAs) exert a profound influence on the body's pathophysiology by intricately shaping the composition of gut bacteria. However, the complex interplay between BAs and gut microbiota has impeded a systematic exploration of their impact on intestinal bacteria. Initially, we investigated the effects of 21 BAs on the growth of 65 gut bacterial strains in vitro. Subsequently, we examined the impact of BAs on the overall composition of intestinal bacteria, both in vivo and in vitro. The results unveiled distinct effects of various BAs on different intestinal strains and their diverse impacts on the composition of gut bacteria. Mechanistically, the inhibition of intestinal strains by BAs occurs through the accumulation of these acids within the strains. The intracellular accumulation of deoxycholic acid (DCA) significantly influenced the growth of intestinal bacteria by impacting ribosome transcription and amino-acid metabolism. The metabolomic analysis underscores the pronounced impact of DCA on amino-acid profiles in both in vivo and in vitro settings. This study not only elucidates the effects of BAs on a diverse range of bacterial strains and their role in shaping the gut microbiota but also reveals underlying mechanisms essential for understanding and maintaining a healthy gut microbiota.

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胆汁酸对肠道细菌的生长、组成和新陈代谢的影响。

胆汁酸（BA）通过复杂地塑造肠道细菌的组成，对人体的病理生理学产生了深远的影响。然而，胆汁酸与肠道微生物群之间复杂的相互作用阻碍了对胆汁酸对肠道细菌影响的系统探索。最初，我们研究了 21 种生物碱对体外 65 种肠道细菌菌株生长的影响。随后，我们研究了 BA 在体内和体外对肠道细菌总体组成的影响。结果揭示了各种生物碱对不同肠道菌株的不同影响，以及它们对肠道细菌组成的不同影响。从机理上讲，BA 对肠道菌株的抑制作用是通过这些酸在菌株内的积累实现的。脱氧胆酸（DCA）在细胞内的积累通过影响核糖体转录和氨基酸代谢而显著影响肠道细菌的生长。代谢组学分析强调了 DCA 在体内和体外环境中对氨基酸谱的明显影响。这项研究不仅阐明了 BAs 对多种细菌菌株的影响及其在塑造肠道微生物群方面的作用，还揭示了了解和维持健康的肠道微生物群所必需的潜在机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology

CiteScore

12.10

自引率

3.30%

发文量

审稿时长

9 weeks

期刊介绍： npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.