A chloroplast localized heavy metal-associated domain containing protein regulates grain calcium accumulation in rice.

IF 14.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Nature Communications Pub Date : 2024-10-27 DOI:10.1038/s41467-024-53648-w

Huan Liu, Cun Lu, Xiang-Qian Liu, Chen-Jin Zhuo, Rong-Jian Luo, Qiu-Tang Huang, Zhong Tang, Chun-Qing Zhao, Mary Lou Guerinot, David E Salt, Fang-Jie Zhao, Xin-Yuan Huang

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Abstract

Calcium (Ca) is an essential mineral nutrient and plays a crucial signaling role in all living organisms. Increasing Ca content in staple foods such as rice is vital for improving Ca nutrition of humans. Here we map a quantitative trait locus that controls Ca concentration in rice grains and identify the causal gene as GCSC1 (Grain Ca and Sr Concentrations 1), which encodes a chloroplast vesicle localized homo-oligomeric protein. GCSC1 exhibits Ca²⁺ transport activity in heterologous assays in yeast and Xenopus laevis oocytes and is involved in the efflux of Ca²⁺ from the chloroplast to the cytosol. Knockout of GCSC1 results in increased chloroplast Ca concentration, lower stomatal conductance in leaves and enhanced Ca allocation to grains. Natural variation in grain Ca concentration is attributed to the variable expression of GCSC1 resulting from its promoter sequence variation. Our study identifies a chloroplast localized heavy metal-associated domain containing protein that regulates chloroplast Ca²⁺ efflux and provides a way to biofortify Ca in rice to benefit human nutrition.

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叶绿体定位的含重金属相关结构域的蛋白质调控水稻谷粒的钙积累。

钙（Ca）是人体必需的矿物质营养素，在所有生物体内都发挥着重要的信号作用。提高大米等主食中的钙含量对改善人类的钙营养至关重要。在这里，我们绘制了一个控制水稻籽粒中钙浓度的数量性状基因座，并确定了其因果基因为 GCSC1（Grain Ca and Sr Concentrations 1），它编码一种叶绿体液泡定位同源异构蛋白。在酵母和爪蟾卵母细胞的异源试验中，GCSC1 表现出 Ca2+ 运输活性，并参与 Ca2+ 从叶绿体向细胞质的外流。基因敲除 GCSC1 会导致叶绿体 Ca 浓度升高、叶片气孔导度降低以及钙在谷粒中的分配增加。谷物钙浓度的自然变化归因于 GCSC1 启动子序列变化导致的不同表达。我们的研究发现了一种叶绿体定位的含重金属相关结构域的蛋白质，它能调节叶绿体 Ca2+ 的外流，为生物强化水稻中的 Ca 提供了一种方法，从而为人类营养带来益处。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Communications Biological Science Disciplines-

CiteScore

24.90

自引率

2.40%

发文量

6928

审稿时长

3.7 months

期刊介绍： Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.