eDNA-Based Survey of Fish Species in Water Bodies Using Loop-Mediated Isothermal Amplification (LAMP) for Application of Developing Automatic Sampler.

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS
Nivedhitha Jothinarayanan, Chau Ha Pham, Frank Karlsen, Lars Eric Roseng
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引用次数: 0

Abstract

The monitoring of species in a habitat is important to ensure biological diversity. Environmental DNA (eDNA) can infer the presence-absence of species and enable rapid action to avoid threatening factors in ecosystems in the case of non-indigenous species. Loop-mediated isothermal amplification (LAMP) assays for molecular amplification are rapidly gaining popularity in species detection, but LAMP remains an underutilized method for eDNA-based monitoring practices. The most effective combination for successful species monitoring may be the collection of eDNA or biological traces collected by nanofiltration followed by LAMP-based species detection initiatives. Here, we used LAMP analysis to detect the eDNA of Esox lucius (Northern Pike), Anguilla anguilla (European Eel), and Salmo salar (Atlantic Salmon) in Borre Lake and in the Drammen River. The selection of species is based on the categories of regionally invasive species, endangered species, and species of least concern. Two target genes were considered for each species and LAMP primers were designed. Our study showed that LAMP is an effective tool for discovering specific fish eDNA (analysis) to maintain aquatic ecosystems.

利用环路介导等温扩增法(LAMP)进行基于 eDNA 的水体鱼类物种调查,以应用正在开发的自动采样器。
监测栖息地中的物种对于确保生物多样性非常重要。环境 DNA(eDNA)可以推断物种的存在与否,并能在非本地物种出现时迅速采取行动,避免生态系统中的威胁因素。用于分子扩增的环介导等温扩增(LAMP)测定法在物种检测中迅速普及,但在基于 eDNA 的监测实践中,LAMP 仍是一种未得到充分利用的方法。成功进行物种监测的最有效组合可能是通过纳滤收集 eDNA 或生物痕量,然后进行基于 LAMP 的物种检测。在这里,我们使用 LAMP 分析方法检测了 Borre 湖和 Drammen 河中 Esox lucius(北方梭子鱼)、Anguilla anguilla(欧洲鳗鱼)和 Salmo salar(大西洋鲑鱼)的 eDNA。物种的选择基于区域入侵物种、濒危物种和最不值得关注物种的类别。每个物种都考虑了两个目标基因,并设计了 LAMP 引物。我们的研究表明,LAMP 是发现特定鱼类 eDNA(分析)以维护水生生态系统的有效工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Methods and Protocols
Methods and Protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)
CiteScore
3.60
自引率
0.00%
发文量
85
审稿时长
8 weeks
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