Miniaturized Pathogen Detection System Using Magnetic Nanoparticles and Microfluidics Technology.

IF 3 3区工程技术 Q2 CHEMISTRY, ANALYTICAL

Micromachines Pub Date : 2024-10-20 DOI:10.3390/mi15101272

Benjamin Garlan, Amine Rabehi, Kieu Ngo, Sophie Neveu, Reza Askari Moghadam, Hamid Kokabi

{"title":"Miniaturized Pathogen Detection System Using Magnetic Nanoparticles and Microfluidics Technology.","authors":"Benjamin Garlan, Amine Rabehi, Kieu Ngo, Sophie Neveu, Reza Askari Moghadam, Hamid Kokabi","doi":"10.3390/mi15101272","DOIUrl":null,"url":null,"abstract":"<p><p>Rapid detection of a biological agent is essential to anticipate a threat to the protection of biodiversity and ecosystems. Our goal is to miniaturize a magnetic pathogen detection system in order to fabricate an efficient and portable system. The detection device is based on flat, multilayer coils associated with microfluidic structures to detect magnetic nanoparticles linked to pathogen agents. One type of immunological diagnosis is based on the measurement of the magnetic sensitivity of magnetic nanoparticles (MNPs), which are markers connected to pathogens. This method of analysis involves the coupling of antibodies or antigen proteins with MNPs. Among the available magnetic techniques, the frequency mixing method has a definite advantage by making it possible to quantify MNPs. An external magnetic field composed of a low- and a high-frequency field is applied to the sample reservoir. Then, the response signal is measured and analyzed. In this paper, magnetic microcoils are implemented on a multilayer Printed Circuit Board (PCB), and a microfluidics microstructure is designed in connection with the planar coils. Simulation software, COMSOL version 5.3, provides an analytical perspective to choose the number of turns in magnetic coils and to understand the effects of changing the shape and dimensions of the microfluidics microstructure.</p>","PeriodicalId":18508,"journal":{"name":"Micromachines","volume":"15 10","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11509726/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Micromachines","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.3390/mi15101272","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Rapid detection of a biological agent is essential to anticipate a threat to the protection of biodiversity and ecosystems. Our goal is to miniaturize a magnetic pathogen detection system in order to fabricate an efficient and portable system. The detection device is based on flat, multilayer coils associated with microfluidic structures to detect magnetic nanoparticles linked to pathogen agents. One type of immunological diagnosis is based on the measurement of the magnetic sensitivity of magnetic nanoparticles (MNPs), which are markers connected to pathogens. This method of analysis involves the coupling of antibodies or antigen proteins with MNPs. Among the available magnetic techniques, the frequency mixing method has a definite advantage by making it possible to quantify MNPs. An external magnetic field composed of a low- and a high-frequency field is applied to the sample reservoir. Then, the response signal is measured and analyzed. In this paper, magnetic microcoils are implemented on a multilayer Printed Circuit Board (PCB), and a microfluidics microstructure is designed in connection with the planar coils. Simulation software, COMSOL version 5.3, provides an analytical perspective to choose the number of turns in magnetic coils and to understand the effects of changing the shape and dimensions of the microfluidics microstructure.

查看原文本刊更多论文

利用磁性纳米粒子和微流体技术的微型病原体检测系统

快速检测生物制剂对于预测对保护生物多样性和生态系统的威胁至关重要。我们的目标是将磁性病原体检测系统微型化，以制造一个高效、便携的系统。该检测装置基于与微流体结构相关的扁平多层线圈，用于检测与病原体相关的磁性纳米粒子。一种免疫学诊断方法是基于测量磁性纳米粒子（MNPs）的磁灵敏度，MNPs 是与病原体相关的标记物。这种分析方法涉及抗体或抗原蛋白与 MNPs 的耦合。在现有的磁性技术中，混频法具有绝对的优势，可以对 MNPs 进行量化。由低频和高频磁场组成的外部磁场被施加到样品池中。然后测量和分析响应信号。本文在多层印刷电路板（PCB）上实现了磁性微线圈，并设计了与平面线圈相关的微流体微结构。COMSOL 5.3 版仿真软件提供了一个分析视角，用于选择磁线圈的匝数，并了解改变微流体微结构的形状和尺寸所产生的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Micromachines NANOSCIENCE & NANOTECHNOLOGY-INSTRUMENTS & INSTRUMENTATION

CiteScore

5.20

自引率

14.70%

发文量

1862

审稿时长

16.31 days

期刊介绍： Micromachines (ISSN 2072-666X) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to micro-scaled machines and micromachinery. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.